Definition of sequence requirements for latency-associated nuclear antigen 1 binding to Kaposi's sarcoma-associated herpesvirus DNA

被引：24

作者：

Srinivasan, V ^{[1
]}

Komatsu, T ^{[1
]}

Ballestas, ME ^{[1
]}

Kaye, KM ^{[1
]}

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Sch Me,Dept Med, Channing Lab, Boston, MA 02115 USA

来源：

JOURNAL OF VIROLOGY | 2004年 / 78卷 / 24期

关键词：

D O I：

10.1128/JVI.78.24.14033-14038.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In latent infection, Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen I (LANA1)-specific binding to KSHV terminal repeat DNA mediates multicopy episome persistence. We now use electrophoretic mobility shift assays to investigate LANA1 binding to its 20-bp cognate sequence. Mutations at positions 6, 7, and 8 (6CCC8) severely reduced LANA1 binding, whereas mutations at other positions only modestly reduced binding. Since 6CCC8 is in the 5' half of an inverted repeat sequence, these results are consistent with an asymmetric role for the inverted repeat in LANA1 binding.

引用

页码：14033 / 14038

页数：6

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