Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

被引：27
作者：
Millan-Plano, Sergio [1 ]
Piedrafita, Eduardo [1 ]
Miana-Mena, Francisco J. [1 ]
Fuentes-Broto, Lorena [1 ]
Martinez-Ballarin, Enrique [1 ]
Lopez-Pingarron, Laura [2 ]
Saenz, Maria A. [1 ]
Garcia, Joaquin J. [1 ]
机构：
[1] Univ Zaragoza, Fac Med, Dept Physiol & Pharmacol, E-50009 Zaragoza, Spain
[2] Univ Zaragoza, Fac Med, Dept Human Anat & Histol, E-50009 Zaragoza, Spain
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 01期
关键词：
melatonin; pinoline; indoleamine; free radical; lipid peroxidation; protein oxidation; synaptosome; INDUCED LIPID-PEROXIDATION; LOW-DENSITY-LIPOPROTEIN; N-ACETYL-SEROTONIN; OXIDATIVE DAMAGE; RAT-BRAIN; HYDROGEN-PEROXIDE; NITRIC-OXIDE; L-TRYPTOPHAN; IN-VITRO; ANTIOXIDANT ACTIVITY;
D O I：
10.3390/ijms11010312
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl3, and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.
引用
收藏
页码：312 / 328
页数：17
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