Development of an enzyme-linked immunosorbent assay for the detection of mebendazole in chicken and mutton

被引：2

作者：

He, Jinxin ^{[1
]}

Zheng, Yan ^{[1
]}

Chen, Xiaorong ^{[1
]}

Li, Na ^{[2
]}

Tang, Fang ^{[1
]}

Huo, Nairui ^{[1
]}

Wang, Jianzhong ^{[1
]}

Gu, Shaopeng ^{[1
]}

机构：

[1] Shanxi Agr Univ, Coll Vet Med, Taigu 030810, Shanxi, Peoples R China

[2] Hangzhou Yuhao Chem Technol Co Ltd, Diaoyu Ind Technol Pk, Hangzhou 311121, Peoples R China

来源：

ANALYTICAL METHODS | 2021年 / 13卷 / 14期

关键词：

IMMUNOASSAY; ELISA;

D O I：

10.1039/d1ay00133g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mebendazole (MBZ), a synthetic benzimidazole, is most widely used for the treatment of intestinal helminthiasis. In the present study, a hapten mimicking the MBZ structure was designed by introducing propanoic acid and coupling to carrier proteins by the active ester method to immunize New Zealand rabbits. A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the analysis of MBZ in food samples. The rabbit IgG based ELISA had a half-maximum inhibition concentration (IC50) of 7.41 ng mL(-1), with a limit of detection of 0.27 ng mL(-1). The ELISA showed negligible cross reactivity (<1%) with structural analogs, including hydroxy-MBZ, albendazole, oxfendazole, fenbendazole, flubendazole and oxfendazole, except the value of 90.5% for amino-MBZ. The average recoveries of MBZ spiked in chicken and mutton muscle samples by the assay ranged from 84.31% to 106.28% and agreed well with those of high-performance liquid chromatography. The above results indicated that the generated anti-MBZ IgG-based ELISA showed promise in routine screening analysis of MBZ residues in food samples.

引用

页码：1740 / 1746

页数：7

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