Real-Time Bioluminescence Imaging of Nitroreductase in Mouse Model

被引：74

作者：

Feng, Ping ^{[1
]}

Zhang, Huateng ^{[3
]}

Deng, Quankun ^{[2
]}

Liu, Wei ^{[2
]}

Yang, Linghui ^{[2
]}

Li, Guobo ^{[2
]}

Chen, Guo ^{[2
]}

Du, Lupei ^{[3
]}

Ke, Bowen ^{[2
]}

Li, Minyong ^{[3
]}

机构：

[1] Sichuan Univ, West China Hosp, Inst Clin Trials, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp, Translat Neurosci Ctr, Lab Anesthesiol & Crit Care Med, Chengdu 610041, Sichuan, Peoples R China

[3] Shandong Univ, Sch Pharm, Dept Med Chem, Key Lab Chem Biol MOE, Jinan 250012, Shandong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 11期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENT-PROBE; HYPOXIA; CELLS; SUBSTRATE; PRODRUGS;

D O I：

10.1021/acs.analchem.6b01160

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nitroreductase (NTR) is an endogenous reductase overexpressed in hypoxic tumors; however, its precise detection in living cells and animals remains a considerable challenge. Herein, we developed three reaction based probes and a related bioluminescence assay for the real-time NTR detection. The high sensitivity and selectivity of probe 3, combined with its remarkable potential of bioluminescence imaging, affords a valuable approach for in vivo imaging of NTR in a tumor model mouse.

引用

页码：5610 / 5614

页数：5

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