Real-Time Bioluminescence Imaging of Nitroreductase in Mouse Model

被引：73

作者：

Feng, Ping ^{[1
]}

Zhang, Huateng ^{[3
]}

Deng, Quankun ^{[2
]}

Liu, Wei ^{[2
]}

Yang, Linghui ^{[2
]}

Li, Guobo ^{[2
]}

Chen, Guo ^{[2
]}

Du, Lupei ^{[3
]}

Ke, Bowen ^{[2
]}

Li, Minyong ^{[3
]}

机构：

[1] Sichuan Univ, West China Hosp, Inst Clin Trials, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp, Translat Neurosci Ctr, Lab Anesthesiol & Crit Care Med, Chengdu 610041, Sichuan, Peoples R China

[3] Shandong Univ, Sch Pharm, Dept Med Chem, Key Lab Chem Biol MOE, Jinan 250012, Shandong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 11期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENT-PROBE; HYPOXIA; CELLS; SUBSTRATE; PRODRUGS;

D O I：

10.1021/acs.analchem.6b01160

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nitroreductase (NTR) is an endogenous reductase overexpressed in hypoxic tumors; however, its precise detection in living cells and animals remains a considerable challenge. Herein, we developed three reaction based probes and a related bioluminescence assay for the real-time NTR detection. The high sensitivity and selectivity of probe 3, combined with its remarkable potential of bioluminescence imaging, affords a valuable approach for in vivo imaging of NTR in a tumor model mouse.

引用

页码：5610 / 5614

页数：5

共 50 条

[1] Real-time bioluminescence imaging of nitroreductase in breast cancer bone metastasis
Lu, Kang
Zhang, Mengxi
Tian, Zuotong
Xiao, Han
RSC CHEMICAL BIOLOGY, 2025, 6 (05): : 754 - 760
[2] Real-time bioluminescence and tomographic imaging of gastric cancer in a novel orthotopic mouse model
Hu, Hao
Liu, Junting
Yao, Liping
Yin, Jipeng
Su, Ning
Liu, Xiangqiang
Cao, Feng
Liang, Jimin
Nie, Yongzhan
Wu, Kaichun
ONCOLOGY REPORTS, 2012, 27 (06) : 1937 - 1943
[3] A turn on fluorescent probe for nitroreductase activity and its application in real-time imaging of tumor hypoxia
Gao, Weijie
Shi, Anyang
Hou, Yunzhuo
Zhang, Peng
Zhang, Qian
Ding, Caifeng
TALANTA, 2025, 290
[4] An activatable water-soluble photoacoustic probe for real-time imaging of endogenous cysteine in the mouse tumor model
Li, Rong
Li, Wenxiu
Chen, Rui
Lin, Weiying
SENSORS AND ACTUATORS B-CHEMICAL, 2021, 347
[5] Real-time in vivo imaging reveals specific nanoparticle target binding in a syngeneic glioma mouse model
Zhang, Wenlong
Chen, Xiangyu
Ding, Dong
Zhang, Guoxin
Zhu, Ziwei
Yang, XingJiu
Li, Mengyuan
Liang, Lijun
Shi, Xudong
Wang, Tie
Gao, Ran
NANOSCALE, 2022, 14 (15) : 5678 - 5688
[6] Real-Time Bioluminescence Imaging of Macroencapsulated Fibroblasts Reveals Allograft Protection in Rhesus Monkeys (Macaca mulatta)
Tarantal, Alice F.
Lee, C. Chang I.
Itkin-Ansari, Pamela
TRANSPLANTATION, 2009, 88 (01) : 38 - 41
[7] A novel low-background nitroreductase fluorescent probe for real-time fluorescence imaging and surgical guidance of thyroid cancer resection
Zhang, Siqi
Ma, Mo
Zhao, Chen
Li, Jingkang
Xu, Lanlan
Zhang, Zihe
Diao, Quanping
Ma, Pinyi
Song, Daqian
BIOSENSORS & BIOELECTRONICS, 2024, 261
[8] Real time detection of live microbes using a highly sensitive bioluminescent nitroreductase probe
Wong, Roger H. F.
Kwong, Thomas
Yau, Kwok-Hei
Au-Yeung, Ho Yu
CHEMICAL COMMUNICATIONS, 2015, 51 (21) : 4440 - 4442
[9] Real-Time Imaging of Cytoplast Acidification in GSNO-induced Mouse Thymocyte early Apoptosis
Liu, Xiaochen
Ma, Wanyun
2010 3RD INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2010), VOLS 1-7, 2010, : 485 - 489
[10] In Vivo Real-Time Multiphoton Imaging of T Lymphocytes in the Mouse Brain After Experimental Stroke
Fumagalli, Stefano
Coles, Jonathan A.
Ejlerskov, Patrick
Ortolano, Fabrizio
Bushell, Trevor J.
Brewer, James M.
De Simoni, Maria-Grazia
Dever, Gary
Garside, Paul
Maffia, Pasquale
Carswell, Hilary V.
STROKE, 2011, 42 (05) : 1429 - 1436

← 1 2 3 4 5 →