Characterization of monoclonal antibodies specific for the Merkel cell polyomavirus capsid

被引：16

作者：

Pastrana, Diana V. ^{[1
]}

Pumphrey, Katherine A. ^{[1
]}

Cuburu, Nicolas ^{[1
]}

Schowalter, Rachel M. ^{[1
]}

Buck, Christopher B. ^{[1
]}

机构：

[1] NCI, Cellular Oncol Lab, Bethesda, MD 20892 USA

来源：

VIROLOGY | 2010年 / 405卷 / 01期

关键词：

MCV; MCPyV; Merkel Cell Carcinoma; Polyomavirus; PyV; HPyV; mAb; Neutralization; VIRUS-LIKE PARTICLES; MEDIATED NEUTRALIZATION; HUMAN-PAPILLOMAVIRUS; CARCINOMA; PROTEINS; DNA; IMMUNOASSAYS; INFECTION; EPITOPES; TISSUES;

D O I：

10.1016/j.virol.2010.06.022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Merkel cell polyomavirus (MCV) has been implicated as a causative agent in Merkel cell carcinoma. Robust polyclonal antibody responses against MCV have been documented in human subjects, but monoclonal antibodies (mAbs) specific for the VP1 capsid protein have not yet been characterized We generated 12 mAbs capable of binding recombinant MCV virus-like particles. The use of a short immunogenic priming schedule was important for production of the mAbs Ten of the 12 mAbs were highly effective for immunofluorescent staining of cells expressing capsid proteins. An overlapping set of 10 mAbs were able to neutralize the infectivity of MCV-based reporter vectors, with 50% effective doses in the low picomolar range. Three mAbs interfered with the binding of MCV virus-like particles to cells This panel of anti-capsid antibodies should provide a useful set of tools for the study of MCV Published by Elsevier Inc

引用

页码：20 / 25

页数：6

共 42 条

[1] Epidemiology of primary Merkel cell carcinoma in the United States [J].