Cloning and Sequence Analysis of Fruit-specific Promoter from Different Genotypes of Tomato

The promoter is an important cis-acting element and an important element of gene engineering expression vectors. In order to explore the difference of 2A11 gene 5 'flanking section of the DNA sequence from different genotypes tomato, the promoter was isolated by PCR from L.Cheesmanii, L.hirsutum, Liaoyuanduoli, Lijiadafen, and Micro-Tom. Primers were designed from the published sequence to amplify two overlapping fragments from an 868bp promoter region. The fragments were cloned into pBS-T vector and then sequenced. The sequences showed some differences between 151bp and 183bp of the translation start site to the published sequences of 2A11 (DG453963.1) promoter. And homology is highly from the wild species and cultivated species (about 90%). The isolation and characterization sequence of fruit-specific promoters will be critical for the manipulation of the nutritional value and quality of fruits by genetic engineering.