β-Glucosidase produced by Moniliophthora perniciosa: Characterization and application in the hydrolysis of sugarcane bagasse

beta-Glucosidases (BGLs) belong to the group of enzymes of cellulases and act in the last stage of cellulose degradation, releasing glucose molecules, eliminating the inhibitory effect of cellobiose. This study focused on the production, characterization, and application of BGL from Moniliophthora perniciosa in the hydrolysis of pretreated sugarcane bagasse (3% NaOH + 6% Na2SO3), with varying enzymatic loads and reaction times. The enzyme showed an optimum pH of 4.5 and 60 degrees C. It was stable at all temperatures analyzed (50-90 degrees C) and retained about 100% of its activity at 50 degrees C after 60 min of incubation. Among the ions analyzed, BaCl2 increased BGL activity 9.04 +/- 1.41 times. The maximum production of reducing sugars (89.15%) was achieved after 48 h with 10 mg of protein.