Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

被引：2

作者：

Aguirre, Carlos ^{[1
]}

Sanchez, Evelyn ^{[2
]}

Olivares, Natalia ^{[3
]}

Hinrichsen, Patricio ^{[1
]}

机构：

[1] INIA La Platina, Inst Invest Agr, Santa Rosa 11,610, Santiago 8831314, Chile

[2] Univ Mayor, Fac Ciencias, Ctr Genom & Bioinformat, Camino Piramide 5750, Santiago, Chile

[3] INIA La Cruz, Inst Invest Agr, Chorrillos 86, La Cruz 2280454, Quillota, Chile

来源：

JOURNAL OF ECONOMIC ENTOMOLOGY | 2021年 / 114卷 / 01期

关键词：

Naupactus cervinus; Naupactus xanthographus; ITS rDNA; citrus industry; Real-Time PCR; MOLECULAR TECHNIQUES; IDENTIFICATION; HYMENOPTERA;

D O I：

10.1093/jee/toaa251

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/mu l to 1 x 10(-6) pg/mu l, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

引用

页码：90 / 99

页数：10

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