Gene expression profile of human lung in a relatively early stage of COPD with emphysema

被引:22
|
作者
Jeong, Ina [1 ]
Lim, Jae-Hyun [2 ]
Oh, Dong Kyu [3 ]
Kim, Woo Jin [4 ,5 ]
Oh, Yeon-Mok [3 ]
机构
[1] Natl Med Ctr, Dept Internal Med, Seoul, South Korea
[2] Seoul Natl Univ, Coll Med, Div Biomed Informat, Biomed Informat Res Ctr, Seoul, South Korea
[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Pulm & Crit Care Med, 88 Olymp Ro 43 Gil, Seoul 05535, South Korea
[4] Kangwon Natl Univ, Sch Med, Kangwon Natl Univ Hosp, Dept Internal Med, Chunchon, South Korea
[5] Kangwon Natl Univ, Sch Med, Kangwon Natl Univ Hosp, Ctr Environm Hlth, Chunchon, South Korea
来源
INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE | 2018年 / 13卷
基金
新加坡国家研究基金会;
关键词
COPD; emphysema; RNA-Seq; transcriptome; gene; GENOME-WIDE ASSOCIATION; DIFFERENTIAL EXPRESSION; PULMONARY; DISEASE; PATHOGENESIS; SEX; REVEALS; ASTHMA; TOPHAT; REPAIR;
D O I
10.2147/COPD.S166812
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Purpose: As only some smokers develop COPD with emphysema, we explored the molecular pathogenesis of early-stage COPD with emphysema using gene expression profiling of human lung tissues. Patients and methods: First, 110 subjects who had smoked more than ten pack-years were classified into three groups: COPD with emphysema, COPD without emphysema, and healthy smokers. COPD and emphysema were confirmed by post-bronchodilator forced expiratory volume in 1 second/forced vital capacity <0.7 and by chest computed tomography. Lung tissues obtained surgically from the 110 subjects were processed and used for RNA-Seq analysis. Results: Among the 110 subjects, 29 had COPD with emphysema, 21 had COPD without emphysema, and 60 were healthy smokers; their mean post-bronchodilator forced expiratory volume in 1 second values were 78%, 80%, and 94%, respectively. Using RNA-Seq, we evaluated 16,676 genes expressed in lung tissues. Among them, 1,226 genes in the COPD with emphysema group and 434 genes in the COPD without emphysema group were differentially expressed genes compared to the expression in healthy smokers. In the COPD with emphysema group, ACER2 and LMAN2L were markedly increased and decreased, respectively. In the COPD without emphysema group, the CHRM3 gene, previously reported to be associated with COPD, and HDAC10 were markedly increased and decreased, respectively. Conclusion: Our study identified differences in gene expression in subjects with COPD according to emphysema status using RNA-Seq transcriptome analysis. These findings may have mechanistic implications in COPD.
引用
收藏
页码:2643 / 2655
页数:13
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