The protective effects of glycyrrhizic acid on heavy metal ions-induced reproductive toxicity in Chinese hamster ovary cells

被引：0
|
作者：
Gao, Liang [1 ]
Liu, Pai [2 ]
Du, Tianqi [1 ]
Yin, Jian [2 ,3 ]
Li, Hong [1 ]
机构：
[1] Nanjing Med Univ, Suzhou Municipal Hosp, Ctr Reprod & Genet, Affiliated Suzhou Hosp, Suzhou 215002, Peoples R China
[2] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou 215163, Peoples R China
[3] Jinan Guo Ke Med Technol Dev Co Ltd, Jinan 250100, Peoples R China
来源：
SCIENCEASIA | 2021年 / 47卷 / 04期
关键词：
Chinese hamster ovary cells; Cd2+; Cu2+; glycyrrhizic acid; oxidative stress; CADMIUM TOXICITY; COPPER; GLUTATHIONE; MATURATION; INTEGRITY; LICORICE; EXPOSURE; EXTRACT; SULFATE;
D O I：
10.2306/scienceasia1513-1874.2021.052
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this work, Chinese hamster ovary (CHO) cells were used to examine the protective effects of glycyrrhizic acid (GA) on the reproductive toxicity of heavy metal ions, including Cd2+ and Cu2+. As a result, both metal ions induced significant toxicity in CHO cells after 48-h treatment, as revealed by a severe decrease in cell viability. GA and glutathione (GSH) largely reduced the toxicity caused by Cd2+ and Cu2+, which could be due to their recovery of GSH levels and superoxide dismutase (SOD) activities in CHO cells. In addition, GA and GSH significantly up-regulated the gene expressions of glutathione S-transferase (gst), sod, and heme oxygenase (ho)-1, which could be another explanation for their protective effects. More importantly, GA exhibited comparable protective effects as GSH but at much lower concentrations (50-100 mu M vs. 500-1000 mu M). Therefore, GA could be effective for the alleviation of reproductive toxicity of Cd2+/Cu2+, which needs further investigation in animal models.
引用
收藏
页码：410 / +
页数：9
相关论文
共 21 条
  • [11] Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
    Anna Ilnicka
    Katarzyna Roszek
    Andrzej Olejniczak
    Michal Komoszynski
    Jerzy P. Lukaszewicz
    Applied Biochemistry and Biotechnology, 2014, 174 : 2153 - 2161
  • [12] Biologically Active Constituents from Salix viminalis Bio-Oil and Their Protective Activity Against Hydrogen Peroxide-Induced Oxidative Stress in Chinese Hamster Ovary Cells
    Ilnicka, Anna
    Roszek, Katarzyna
    Olejniczak, Andrzej
    Komoszynski, Michal
    Lukaszewicz, Jerzy P.
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2014, 174 (06) : 2153 - 2161
  • [13] Hypochlorous acid-induced oxidative stress in Chinese hamster B14 cells: viability, DNA and protein damage and the protective action of melatonin
    Zavodnik, IB
    Lapshina, EA
    Zavodnik, LB
    Labieniec, M
    Bryszewska, M
    Reiter, RJ
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2004, 559 (1-2) : 39 - 48
  • [14] Potential Protective Effects of Spirulina (Spirulina platensis) against In Vitro Toxicity Induced by Heavy Metals (Cadmium, Mercury, and Lead) on SH-SY5Y Neuroblastoma Cells
    Mallamaci, Rosanna
    Storelli, Maria Maddalena
    Barbarossa, Alexia
    Messina, Giovanni
    Valenzano, Anna
    Meleleo, Daniela
    Hau, Peter
    Constantin-Teodosiu, Dumitru
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (23)
  • [15] Evaluation of the Potential Protective Effect of Ellagic Acid against Heavy Metal (Cadmium, Mercury, and Lead) Toxicity in SH-SY5Y Neuroblastoma Cells
    Mallamaci, Rosanna
    Barbarossa, Alexia
    Carocci, Alessia
    Meleleo, Daniela
    FOODS, 2024, 13 (03)
  • [16] Protective Effects of Mitochondrial Uncoupling Protein 2 against Aristolochic Acid I-Induced Toxicity in HK-2 Cells
    Feng, Chen
    Anger, Etienne Empweb
    Zhang, Xiong
    Su, Shengdi
    Su, Chenlin
    Zhao, Shuxin
    Yu, Feng
    Li, Ji
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (07)
  • [17] Synthesis, physicochemical properties, and protective effects of a novel water-soluble tetrahydrocurcumin-diglutaric acid prodrug on ethanol-induced toxicity in HepG2 cells
    Nattapong Jongjitphisut
    Rianthong Phumsuay
    Worathat Thitikornpong
    Paitoon Rashatasakhon
    Chawanphat Muangnoi
    Opa Vajragupta
    Pornchai Rojsitthisak
    Journal of Pharmaceutical Investigation, 2022, 52 : 477 - 487
  • [18] Synthesis, physicochemical properties, and protective effects of a novel water-soluble tetrahydrocurcumin-diglutaric acid prodrug on ethanol-induced toxicity in HepG2 cells
    Jongjitphisut, Nattapong
    Phumsuay, Rianthong
    Thitikornpong, Worathat
    Rashatasakhon, Paitoon
    Muangnoi, Chawanphat
    Vajragupta, Opa
    Rojsitthisak, Pornchai
    JOURNAL OF PHARMACEUTICAL INVESTIGATION, 2022, 52 (04) : 477 - 487
  • [19] Protective Effects of Gallic Acid Against NiSO4-Induced Toxicity Through Down-Regulation of the Ras/ERK Signaling Pathway in Beas-2B Cells
    An, Xuejun
    Zhou, Aijia
    Yang, Yue
    Wang, Yue
    Xin, Rui
    Tian, Chao
    Wu, Yonghui
    MEDICAL SCIENCE MONITOR, 2016, 22 : 3446 - 3454
  • [20] Caffeine-induced release of intracellular Ca2+ from Chinese hamster ovary cells expressing skeletal muscle ryanodine receptor - Effects on full-length and carboxyl-terminal portion of Ca2+ release channels
    Manjunatha, B
    Zhao, JY
    Zang, WJ
    Balke, CW
    Takeshima, H
    Wier, WG
    Ma, JJ
    JOURNAL OF GENERAL PHYSIOLOGY, 1997, 110 (06) : 749 - 762
123