Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to "Teflon" Proteins

被引：52

作者：

Merkel, Lars ^{[1
]}

Schauer, Melina ^{[1
]}

Antranikian, Garabed ^{[2
]}

Budisa, Nediljko ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

[2] Hamburg Univ Technol, Inst Tech Microbiol, D-21073 Hamburg, Germany

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 11期

关键词：

enzyme activity; lipases; noncanonical amino acids; protein engineering; sense-codon reassignment; ATOMIC MUTATIONS; GENETIC-CODE; ANNEXIN-V; STABILITY;

D O I：

10.1002/cbic.201000295

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1505 / 1507

页数：3

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