Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to "Teflon" Proteins

被引：52
|
作者：
Merkel, Lars [1 ]
Schauer, Melina [1 ]
Antranikian, Garabed [2 ]
Budisa, Nediljko [1 ]
机构：
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
[2] Hamburg Univ Technol, Inst Tech Microbiol, D-21073 Hamburg, Germany
来源：
CHEMBIOCHEM | 2010年 / 11卷 / 11期
关键词：
enzyme activity; lipases; noncanonical amino acids; protein engineering; sense-codon reassignment; ATOMIC MUTATIONS; GENETIC-CODE; ANNEXIN-V; STABILITY;
D O I：
10.1002/cbic.201000295
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：1505 / 1507
页数：3
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