Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to "Teflon" Proteins

被引：52
|
作者：
Merkel, Lars [1 ]
Schauer, Melina [1 ]
Antranikian, Garabed [2 ]
Budisa, Nediljko [1 ]
机构：
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
[2] Hamburg Univ Technol, Inst Tech Microbiol, D-21073 Hamburg, Germany
来源：
CHEMBIOCHEM | 2010年 / 11卷 / 11期
关键词：
enzyme activity; lipases; noncanonical amino acids; protein engineering; sense-codon reassignment; ATOMIC MUTATIONS; GENETIC-CODE; ANNEXIN-V; STABILITY;
D O I：
10.1002/cbic.201000295
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
(Chemical Equation Presented) Nonstick proteins? We report the successful residue-specific fluorination of 24 positions in a lipase by simultaneous incorporation of three different noncanonical amino acids. The enzyme produced in a polyauxotrophic E. coli strain structurally tolerates all fluorine atoms and retains its catalytic activity very well. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：1505 / 1507
页数：3
相关论文
共 50 条
  • [1] Biosynthetic Incorporation of Fluorinated Amino Acids into Peptides and Proteins
    Fraser, Samuel A.
    Easton, Christopher J.
    AUSTRALIAN JOURNAL OF CHEMISTRY, 2015, 68 (01) : 9 - 12
  • [2] In Vivo Incorporation of Multiple Noncanonical Amino Acids into Proteins
    Hoesl, Michael G.
    Budisa, Nediljko
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (13) : 2896 - 2902
  • [3] Incorporation of nonstandard amino acids into proteins: principles and applications
    Wang, Tianwen
    Liang, Chen
    Xu, Hongjv
    An, Yafei
    Xiao, Sha
    Zheng, Mengyuan
    Liu, Lu
    Nie, Lei
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2020, 36 (04):
  • [4] Site-specific incorporation of unnatural amino acids into proteins
    Stromgaard, A
    Jensen, AA
    Stromgaard, K
    CHEMBIOCHEM, 2004, 5 (07) : 909 - 916
  • [5] Incorporation of non-canonical amino acids into proteins in yeast
    Wiltschi, Birgit
    FUNGAL GENETICS AND BIOLOGY, 2016, 89 : 137 - 156
  • [6] Selection, Addiction and Catalysis: Emerging Trends for the Incorporation of Noncanonical Amino Acids into Peptides and Proteins in Vivo
    Mayer, Clemens
    CHEMBIOCHEM, 2019, 20 (11) : 1357 - 1364
  • [7] Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition
    Dai, Zhen
    Tan, Linzhi
    Su, Yeyu
    Zhao, Kerui
    Tian, Yushun
    Liu, Yu
    Liu, Tao
    CHINESE CHEMICAL LETTERS, 2024, 35 (05)
  • [8] Advances and Challenges in Cell-Free Incorporation of Unnatural Amino Acids Into Proteins
    Gao, Wei
    Cho, Eunhee
    Liu, Yingying
    Lu, Yuan
    FRONTIERS IN PHARMACOLOGY, 2019, 10
  • [9] Enhancing the incorporation of lysine derivatives into proteins with methylester forms of unnatural amino acids
    Zhou, Han
    Cheung, Jenny W.
    Carpenter, Tomaya
    Jones, Stacey K., Jr.
    Luong, Nhu H.
    Tran, Nhi C.
    Jacobs, Savannah E.
    Liyanage, Sahan A. Galbada
    Cropp, T. Ashton
    Yin, Jun
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2020, 30 (02)
  • [10] Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis
    Shozen, Naoki
    Iijima, Issei
    Hohsaka, Takahiro
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2009, 19 (17) : 4909 - 4911
12345