Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

被引：12

作者：

Gatera, Vesara A. ^{[1
,2
]}

Lesmana, Ronny ^{[3
,4
,5
]}

Musfiroh, Ida ^{[6
]}

Judistiani, Raden Tina D. ^{[7
]}

Setiabudiawan, Budi ^{[8
]}

Abdulah, Rizky ^{[1
,5
]}

机构：

[1] Univ Padjadjaran, Dept Pharmacol & Clin Pharm, Fac Pharm, Bandung, Indonesia

[2] Singaperbangsa Univ, Fac Hlth, Dept Pharm, Karawang, Indonesia

[3] Univ Padjadjaran, Div Physiol, Dept Biomed Sci, Fac Med, Bandung, Indonesia

[4] Univ Padjadjaran, Div Biol Act, Cent Lab, Bandung, Indonesia

[5] Univ Padjadjaran, Ctr Excellence Higher Educ Pharmaceut Care Innova, Bandung, Indonesia

[6] Univ Padjadjaran, Dept Pharmaceut Anal & Med Chem, Fac Pharm, Bandung, Indonesia

[7] Univ Padjadjaran, Dept Publ Hlth, Fac Med, Bandung, Indonesia

[8] Univ Padjadjaran, Dept Child Hlth, Fac Med, Bandung, Indonesia

来源：

MEDICAL SCIENCE MONITOR BASIC RESEARCH | 2021年 / 27卷

关键词：

1,25-dihydroxyvitamin D; Cytokines; Inflammation; Lipopolysaccharides; Lung; NF-KAPPA-B; TOLL-LIKE RECEPTOR-4; LUNG INFLAMMATION; EXPRESSION; INFLUENZA; ASTHMA;

D O I：

10.12659/MSMBR.931481

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Studies have shown that lung inflammation affects lung function, with life-threatening results. Vitamin D may play an important role in inhibiting inflammatory cytokines. Vitamin D deficiency is related to several lung problems, including respiratory distress syndrome, alveolar inflammation, epithelial damage, and hypoxia. Few studies have evaluated the benefits of vitamin D in preventing inflammation in alveolar cells. Material/Methods: We developed a cell inflammation model induced by lipopolysaccharide (LPS) treatment. The effects of vitamin D on LPS-induced inflammation in A549 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anti-inflammatory mechanism of vitamin D was evaluated using western blot analysis. Results: Our results indicated that vitamin D promoted A549 cell survival following LPS-induced inflammation by down regulating nuclear factor nuclear factor kappa light chain enhancer of activated B cells, tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and IL-12. Conclusions: Our results indicated that vitamin D has the potential to manage lung inflammation, although further studies are needed.

引用

页数：8

共 45 条

[31] Interleukin-1 Beta Induces an Inflammatory Phenotype in Human Aortic Valve Interstitial Cells Through Nuclear Factor Kappa Beta [J].