DNA-triplex stabilizing properties of 8-aminoguanine

被引：35
作者：
Soliva, R
García, RG
Blas, JR
Eritja, R
Asensio, JL
González, C
Luque, FJ
Orozco, M
机构：
[1] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain
[2] European Mol Biol Lab, D-69117 Heidelberg, Germany
[3] CSIC, Inst Estructura Mat, E-28006 Madrid, Spain
[4] CSIC, Inst Quim Organ, E-28006 Madrid, Spain
[5] Univ Barcelona, Fac Farm, Dept Fisicoquim, E-08028 Barcelona, Spain
来源：
NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 22期
关键词：
D O I：
10.1093/nar/28.22.4531
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A DNA-triplex stabilizing purine (8-aminoguanine) is designed based on molecular modeling and synthesized, The substitution of guanine by 8-aminoguanine largely stabilizes the tripler both at neutral and acidic pH, as suggested by molecular dynamics and thermodynamic integration calculations, and demonstrated by melting experiments, NMR experiments confirm the tripler-stabilizing properties of 8-aminoguanine and demonstrate that few changes are found in the structure of the tripler due to the presence of this modified base.
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页码：4531 / 4539
页数：9
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