Transcriptomic profiling of urine extracellular vesicles reveals alterations of CDH3 in prostate cancer

被引：54

作者：

Royo, Felix ^{[1
,2
]}

Zuniga-Garcia, Patricia ^{[1
]}

Torrano, Veronica ^{[1
]}

Loizaga, Ana ^{[3
]}

Sanchez-Mosquera, Pilar ^{[1
]}

Ugalde-Olano, Aitziber ^{[4
]}

Gonzalez, Esperanza ^{[1
]}

Cortazar, Ana R. ^{[1
]}

Palomo, Laura ^{[1
]}

Fernandez-Ruiz, Sonia ^{[1
]}

Lacasa-Viscasillas, Isabel ^{[3
]}

Berdasco, Maria ^{[5
]}

Sutherland, James D. ^{[1
]}

Barrio, Rosa ^{[1
]}

Zabala-Letona, Amaia ^{[1
]}

Martin-Martin, Natalia ^{[1
]}

Arruabarrena-Aristorena, Amaia ^{[1
]}

Valcarcel-Jimenez, Lorea ^{[1
]}

Caro-Maldonado, Alfredo ^{[1
]}

Gonzalez-Tampan, Jorge ^{[3
]}

Cachi-Fuentes, Guido ^{[3
]}

Esteller, Manel ^{[5
]}

Aransay, Ana M. ^{[1
,2
]}

Unda, Miguel ^{[3
]}

Falcon-Perez, Juan M. ^{[1
,2
,6
]}

Carracedo, Arkaitz ^{[1
,6
,7
]}

机构：

[1] CIC BioGUNE, Bizkaia Technol Pk, Biscay, Spain

[2] Ctr Invest Biomed Red Enfermedades Hepat & Digest, Barcelona, Spain

[3] Basurto Univ Hosp, Dept Urol, Bilbao, Spain

[4] Basurto Univ Hosp, Dept Pathol, Bilbao, Spain

[5] Bellvitge Biomed Res Inst IDIBELL, Canc Epigenet & Biol Program, Barcelona, Spain

[6] Basque Fdn Sci, Ikerbasque, Bizkaia, Spain

[7] Univ Basque Country UPV EHU, Biochem & Mol Biol Dept, Bizkaia, Spain

来源：

ONCOTARGET | 2016年 / 7卷 / 06期

基金：

欧洲研究理事会;

关键词：

extracellular vesicles; exosomes; prostate cancer; urine biomakers; P-CADHERIN; EPITHELIAL MARKER; EXOSOMES; P63; BIOMARKERS; IDENTIFICATION; DEMETHYLATION; EXPRESSION; PROTEOMICS; INDICATOR;

D O I：

10.18632/oncotarget.6899

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Extracellular vesicles (EV) are emerging structures with promising properties for intercellular communication. In addition, the characterization of EV in biofluids is an attractive source of non-invasive diagnostic, prognostic and predictive biomarkers. Here we show that urinary EV (uEV) from prostate cancer (PCa) patients exhibit genuine and differential physical and biological properties compared to benign prostate hyperplasia (BPH). Importantly, transcriptomics characterization of uEVs led us to define the decreased abundance of Cadherin 3, type 1 (CDH3) transcript in uEV from PCa patients. Tissue and cell line analysis strongly suggested that the status of CDH3 in uEVs is a distal reflection of changes in the expression of this cadherin in the prostate tumor. CDH3 was negatively regulated at the genomic, transcriptional, and epigenetic level in PCa. Our results reveal that uEVs could represent a non-invasive tool to inform about the molecular alterations in PCa.

引用

页码：6835 / 6846

页数：12

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