Mechanics and imaging of single DNA molecules

被引：8
作者：
Hegner, M [1 ]
Grange, W [1 ]
机构：
[1] Univ Basel, Inst Phys, NCCR Nanoscale Sci, CH-4056 Basel, Switzerland
来源：
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY | 2002年 / 23卷 / 5-6期
关键词：
D O I：
10.1023/A:1023446103620
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We review recent experiments that have revealed mechanical properties of single DNA molecules using advanced manipulation and force sensing techniques (scanning force microscopy (SFM), optical or magnetic tweezers, microneedles). From such measurements, intrinsic relevant parameters (persistence length, stretch modulus) as well as their dependence on external parameters (non-physiological conditions, coating with binding agents or proteins) are obtained on a single-molecule level. In addition, imaging of DNA molecules using SFM is presented.
引用
收藏
页码：367 / 375
页数：9
相关论文
共 74 条
  • [1] Stretched and overwound DNA forms a Pauling-like structure with exposed bases
    Allemand, JF
    Bensimon, D
    Lavery, R
    Croquette, V
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) : 14152 - 14157
  • [2] Direct atomic force microscope imaging of EcoRI endonuclease site specifically bound to plasmid DNA molecules
    Allison, DP
    Kerper, PS
    Doktycz, MJ
    Spain, JA
    Modrich, P
    Larimer, FW
    Thundat, T
    Warmack, RJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (17) : 8826 - 8829
  • [3] Mapping individual cosmid DNAs by direct AFM imaging
    Allison, DP
    Kerper, PS
    Doktycz, MJ
    Thundat, T
    Modrich, P
    Larimer, FW
    Johnson, DK
    Hoyt, PR
    Mucenski, ML
    Warmack, RJ
    [J]. GENOMICS, 1997, 41 (03) : 379 - 384
  • [4] Ionic effects on the elasticity of single DNA molecules
    Baumann, CG
    Smith, SB
    Bloomfield, VA
    Bustamante, C
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) : 6185 - 6190
  • [5] Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers
    Bennink, ML
    Leuba, SH
    Leno, GH
    Zlatanova, J
    de Grooth, BG
    Greve, J
    [J]. NATURE STRUCTURAL BIOLOGY, 2001, 8 (07) : 606 - 610
  • [6] STRETCHING DNA WITH A RECEDING MENISCUS - EXPERIMENTS AND MODELS
    BENSIMON, D
    SIMON, AJ
    CROQUETTE, V
    BENSIMON, A
    [J]. PHYSICAL REVIEW LETTERS, 1995, 74 (23) : 4754 - 4757
  • [7] Unzipping DNA with optical tweezers: high sequence sensitivity and force flips
    Bockelmann, U
    Thomen, P
    Essevaz-Roulet, B
    Viasnoff, V
    Heslot, F
    [J]. BIOPHYSICAL JOURNAL, 2002, 82 (03) : 1537 - 1553
  • [8] Molecular stick-slip motion revealed by opening DNA with piconewton forces
    Bockelmann, U
    EssevazRoulet, B
    Heslot, F
    [J]. PHYSICAL REVIEW LETTERS, 1997, 79 (22) : 4489 - 4492
  • [9] DIRECT MEASUREMENT OF HYDROGEN-BONDING IN DNA NUCLEOTIDE BASES BY ATOMIC-FORCE MICROSCOPY
    BOLAND, T
    RATNER, BD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) : 5297 - 5301
  • [10] Estimating the persistence length of a worm-like chain molecule from force-extension measurements
    Bouchiat, C
    Wang, MD
    Allemand, JF
    Strick, T
    Block, SM
    Croquette, V
    [J]. BIOPHYSICAL JOURNAL, 1999, 76 (01) : 409 - 413
12345678