Xylose isomerase. activity influences xylose fermentation with recombinant Saccharomyces cerevisiae strains expressing mutated xylA from Thermus thermophilus

被引：37
作者：
Lönn, A
Träff-Bjerre, KL
Otero, RRC
van Zyl, WH
Hahn-Hägerdal, B
机构：
[1] Lund Univ, Dept Appl Microbiol, S-22100 Lund, Sweden
[2] Univ Stellenbosch, Inst Biotechnol, ZA-7202 Matieland, South Africa
[3] Univ Stellenbosch, Dept Microbiol, ZA-7202 Matieland, South Africa
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2003年 / 32卷 / 05期
关键词：
xylose; xylose isomerase; xylulokinase; xylose fermentation; Saccharomyces cerevisiae; GRE3;
D O I：
10.1016/S0141-0229(03)00024-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Three xylose isomerase enzymes (XI) [Eur. J. Biochem. 269 (2002) 157] encoded by mutated xylA genes from Thermus thermophilus were produced at two different levels in Saccharomyces cerevisiae; xylA genes were chromosomally integrated and expressed from multicopy plasmids, respectively. An extra copy of the endogenous xylulokinase gene (XKSI) was chromosomally integrated and the aldose reductase (AR) GRE3 gene was deleted. Ethanol was formed from xylose only when xylA was expressed from multicopy plasmids and when the specific XI activity was higher than 30 mU/mg protein. Deletion of the GRE3 gene was crucial for ethanol formation, possibly because reduced xylitol formation caused less inhibition of XI. (C) 2003 Elsevier Science Inc. All rights reserved.
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页码：567 / 573
页数：7
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