Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine

被引：119
作者：
Gill, H. S. [1 ]
Soderholm, J. [2 ]
Prausnitz, M. R. [1 ,3 ]
Sallberg, M. [2 ]
机构：
[1] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA
[2] Karolinska Univ Hosp Huddinge, Div Clin Microbiol, Karolinska Inst, Stockholm, Sweden
[3] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
来源：
GENE THERAPY | 2010年 / 17卷 / 06期
基金：
美国国家卫生研究院;
关键词：
cellular immune response; DNA-coated microneedle; DNA vaccine; hepatitis C virus; microfabricated needle; TRANSDERMAL DELIVERY; IN-VIVO; IMMUNIZATION; GENE; IMMUNOGENICITY; DEVICE;
D O I：
10.1038/gt.2010.22
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination. Gene Therapy (2010) 17, 811-814; doi: 10.1038/gt.2010.22; published online 4 March 2010
引用
收藏
页码：811 / 814
页数：4
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