Interactions of bile salt micelles and colipase studied through intermolecular nOes

被引：15

作者：

Dominguez, C ^{[1
]}

Sebban-Kreuzer, C ^{[1
]}

Bornet, O ^{[1
]}

Kerfelec, B ^{[1
]}

Chapus, C ^{[1
]}

Guerlesquin, F ^{[1
]}

机构：

[1] IBSM, CNRS, Unite Bioenerget & Ingn Prot, F-13204 Marseille 20, France

来源：

FEBS LETTERS | 2000年 / 482卷 / 1-2期

关键词：

colipase; bile salt; micelle; nuclear magnetic resonance; intermolecular nuclear Overhauser effect;

D O I：

10.1016/S0014-5793(00)02034-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Colipase is a small protein (10 kDa), which acts as a protein cofactor for the pancreatic lipase, Various models of the activated ternary complex; (lipase-colipase-bile salt micelles) have been proposed using detergent micelles, but no structural information has been established with bile salt micelles. We hare investigated the organization of sodium taurodeoxycholate (NaTDC) micelles and their interactions with pig and horse colipases by homonuclear nuclear magnetic resonance (NMR) spectroscopy. The NR;IR data supply evidence that the folding of horse colipase is similar to that already described for pig colipase, Intermolecular nuclear Overhauser effects have shown that two conserved aromatic residues interact with NaTDC micelles, (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.

引用

页码：109 / 112

页数：4

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