Displacement and strain mapping for osteocytes under fluid shear stress using digital holographic microscopy and digital image correlation

被引：8

作者：

Cao, Runyu ^{[1
]}

Xiao, Wen ^{[1
]}

Pan, Feng ^{[1
]}

Tian, Ran ^{[2
]}

Wu, Xintong ^{[2
]}

Sun, Lianwen ^{[2
]}

机构：

[1] Beihang Univ, Sch Instrumentat & Optoelect Engn, Key Lab Precis Optomechatron Technol, Beijing 100083, Peoples R China

[2] Beihang Univ, Sch Biol Sci & Med Engn, Beijing Adv Innovat Ctr Biomed Engn, Minist Educ,Key Lab Biomech & Mechanobiol, Beijing 100083, Peoples R China

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 04期

基金：

中国国家自然科学基金;

关键词：

LABEL-FREE ANALYSIS; SIMULATED MICROGRAVITY; CELL;

D O I：

10.1364/BOE.418418

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteocytes, as the mechano-sensors in bone, are always subjected to fluid shear stress (FSS) from the surrounding matrix. Quantification of FSS-induced cellular deformation is significant for clarifying the ?perceive and transmit? process of cellular mechanotransduction. In this research, a label-free displacement and strain mapping method based on digital holographic microscopy (DHM) and digital image correlation (DIC) is introduced. The method, which is termed DHM-DIC, innovatively utilizes surface features extracted from holographic phase images instead of speckles as the metric for DIC searching. Simulation results on a hemisphere validate the feasibility of DHM-DIC. Displacement and strain maps of living osteocytes under 1.5 Pa FSS are evaluated from DHM-DIC and present good agreement with our previous finite element modeling results.

引用

页码：1922 / 1933

页数：12

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