Construction of surface-engineered arming yeasts with fluorescent protein sensors in response to environmental changes

Although biosensors using microorganisms play important roles in bioprocesses, sensing abilities in view of versatility are quite limited because the sensing system depends on only a single metabolic reaction. Moreover, for withdrawing of the information from these systems, there are a lot of steps including invasive methods. Appearance of gene-engineered cells made us possible to draw the several information from cells with non-invasive manner at real time. First of all, Aequorea victoria GFP was successfully displayed as its active form on Saccharomyces cerevisiae depending on promoters. Secondly, GFP and its variant could be expressed on same cell surface in response to glucose concentration by the different colors. That cell was able to put out the intra- and extracellular informations. Thirdly, quantitative systems of fluorescent protein-displaying cell were examined and confirmed as useful for basic and practical studies. We have developed novel systems of non-invasive sensing of metabolites by using the surface-engineered yeast cells with fluorescent proteins.