Serological and molecular variability of watermelon mosaic virus (genus Potyvirus)

被引:48
作者
Desbiez, C. [1 ]
Costa, C. [1 ]
Wipf-Scheibel, C. [1 ]
Girard, M. [1 ]
Lecoq, H. [1 ]
机构
[1] INRA, Pathol Vegetale Stn, F-84143 Montfavet, France
关键词
D O I
10.1007/s00705-006-0899-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Watermelon mosaic virus (WMV, genus Potyvirus) is very common in cucurbits worldwide, but its variability has been little studied. In France, where WMV has been known since 1974, unusually severe symptoms on zucchini squash have been found to be associated with WMV since 1999. We have developed serological and molecular tools to study WMV variability and the origin of severe strains. Eight monoclonal antibodies were obtained, characterized by epitope mapping, and used to assess the serological variability of 42 isolates from different countries. Sequence analysis based on the NIb-CP region revealed an important variability, with three distinct molecular groups. These analyses also suggested frequent intraspecific recombination in WMV.
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收藏
页码:775 / 781
页数:7
相关论文
共 25 条
[1]   The complete nucleotide sequence of a Pakistani isolate of Watermelon mosaic virus provides further insights into the taxonomic status in the bean common mosaic virus subgroup [J].
Ali, Asad ;
Natsuaki, Tomohide ;
Okuda, Seiichi .
VIRUS GENES, 2006, 32 (03) :307-311
[2]   ANTIGENIC ANALYSIS OF POTATO VIRUS-A PARTICLES AND COAT PROTEIN [J].
ANDREEVA, L ;
JARVEKULG, L ;
RABENSTEIN, F ;
TORRANCE, L ;
HARRISON, BD ;
SAARMA, M .
ANNALS OF APPLIED BIOLOGY, 1994, 125 (02) :337-348
[3]  
ARTEAGA MPL, 1976, ANN PHYTOPATHOL, V8, P347
[4]   AMINO-ACID SUBSTITUTIONS IN THE COAT PROTEIN RESULT IN LOSS OF INSECT TRANSMISSIBILITY OF A PLANT-VIRUS [J].
ATREYA, PL ;
ATREYA, CD ;
PIRONE, TP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7887-7891
[5]   On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus [J].
Bateson, MF ;
Lines, RE ;
Revill, P ;
Chaleeprom, W ;
Ha, CV ;
Gibbs, AJ ;
Dale, JL .
JOURNAL OF GENERAL VIROLOGY, 2002, 83 :2575-2585
[6]   The nucleotide sequence of Watermelon mosaic virus (WMV, Potyvirus) reveals interspecific recombination between two related potyviruses in the 5′ part of the genome [J].
Desbiez, C ;
Lecoq, H .
ARCHIVES OF VIROLOGY, 2004, 149 (08) :1619-1632
[7]   Biological and molecular variability of zucchini yellow mosaic virus on the island of Martinique [J].
Desbiez, C ;
WipfScheibel, C ;
Granier, F ;
Robaglia, C ;
Delaunay, T ;
Lecoq, H .
PLANT DISEASE, 1996, 80 (02) :203-207
[8]   Biological and serological variability, evolution and molecular epidemiology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) with special reference to Caribbean islands [J].
Desbiez, C ;
Wipf-Scheibel, C ;
Lecoq, H .
VIRUS RESEARCH, 2002, 85 (01) :5-16
[9]   Identification of immunogenic hot spots within plum pox potyvirus capsid protein for efficient antigen presentation [J].
Fernández-Fernández, MR ;
Martínez-Torrecuadrada, JL ;
Roncal, F ;
Domínguez, E ;
García, JA .
JOURNAL OF VIROLOGY, 2002, 76 (24) :12646-12653
[10]   THE USE OF 3' NON-CODING NUCLEOTIDE-SEQUENCES IN THE TAXONOMY OF POTYVIRUSES - APPLICATION TO WATERMELON MOSAIC VIRUS-2 AND SOYBEAN MOSAIC VIRUS-N [J].
FRENKEL, MJ ;
WARD, CW ;
SHUKLA, DD .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :2775-2783