Electroacupuncture relieves neuropathic pain via upregulation of glutamate transporters in the spinal cord of rats

被引：28

作者：

Zeng, Jie ^{[1
]}

Cui, Lu-ying ^{[1
]}

Feng, Yan ^{[1
]}

Ding, Ming-xing ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2016年 / 620卷

基金：

中国国家自然科学基金;

关键词：

Neuropathic pain; Electroacupuncture; GLAST; GLT-1; l-trans-Pyrrolidine-2-4-dicarboxylate; NERVE INJURY; RECEPTOR ANTAGONISTS; GLIAL ACTIVATION; MODEL; TRANSMISSION; SUPPRESSION; PHYSIOLOGY;

D O I：

10.1016/j.neulet.2016.03.041

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Glutamate transports (GTs), the only vehicle for removal of glutamate from the extracellular fluid, is reported to be related to chronic pain. To investigate whether the glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) participate in electroacupuncture (EA) analgesia, the EA effect was observed with paw withdraw threshold in a rat model of spared nerve injury. The expression levels of GLAST and GLT-1 were determined with Western Blot and RT-PCR. The results showed significantly upregulated GLAST and GLT-1, along with the relieved pain behaviors after EA treatment. In addition, intrathecal injection of GTs inhibitor, L-trans-pyrrolidine-2-4-dicarboxylate, attenuated the EA-induced analgesic effect. The experiment demonstrates that EA can increase the GTs of neuropathic pain rats, which might be one of the mechanisms underlying its effectiveness in the neuropathic pain. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

引用

页码：38 / 42

页数：5

共 36 条

[1] GLUTAMATE IMMUNOCYTOCHEMISTRY IN THE DORSAL HORN AFTER INJURY OR STIMULATION OF THE SCIATIC-NERVE OF RATS [J].

ALGHOUL, WM ;

VOLSI, GL ;

WEINBERG, RJ ;

RUSTIONI, A .

BRAIN RESEARCH BULLETIN, 1993, 30 (3-4) :453-459

[2] Pain [J].

Basbaum, AI ;

Woolf, CJ .

CURRENT BIOLOGY, 1999, 9 (12) :R429-R431

[3] A PERIPHERAL MONONEUROPATHY IN RAT THAT PRODUCES DISORDERS OF PAIN SENSATION LIKE THOSE SEEN IN MAN [J].

BENNETT, GJ ;

XIE, YK .

PAIN, 1988, 33 (01) :87-107

[4] Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats [J].

Cheng, Li-Li ;

Ding, Ming-Xing ;

Wei, Jia ;

Wu, Yi-Qiang ;

Qiu, Zheng-Ying ;

Chen, Jian-Guo ;

Liu, Dong-Ming ;

Hu, Chang-Min ;

Hu, Man-Li ;

Shah, Zahir ;

Wang, Qiong .

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2013, 2013

[5] Glutamate uptake [J].

Danbolt, NC .

PROGRESS IN NEUROBIOLOGY, 2001, 65 (01) :1-105

[6]

DAVIES P, 1984, ANNU REV IMMUNOL, V2, P335, DOI 10.1146/annurev.immunol.2.1.335

[7] Spared nerve injury: an animal model of persistent peripheral neuropathic pain [J].

Decosterd, I ;

Woolf, CJ .

PAIN, 2000, 87 (02) :149-158

[8] A CURE FOR WIND UP - NMDA RECEPTOR ANTAGONISTS AS POTENTIAL ANALGESICS [J].

DICKENSON, AH .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1990, 11 (08) :307-309

[9] The pharmacology of excitatory and inhibitory amino acid-mediated events in the transmission and modulation of pain in the spinal cord [J].

Dickenson, AH ;

Chapman, V ;

Green, GM .

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM, 1997, 28 (05) :633-638

[10] Targeting Astrocyte Signaling for Chronic Pain [J].

Gao, Yong-Jing ;

Ji, Ru-Rong .

NEUROTHERAPEUTICS, 2010, 7 (04) :482-493

← 1 2 3 4 →