Light-induced two-dimensional FT-IR spectroscopy of BacterioRhodopsin

被引：2
|
作者：
Kosters, PGH [1 ]
de Vries, AHB [1 ]
Kooyman, RPH [1 ]
机构：
[1] Univ Twente, Dept Appl Phys, Biointerface Grp, NL-7500 AE Enschede, Netherlands
来源：
APPLIED SPECTROSCOPY | 2000年 / 54卷 / 11期
关键词：
BacterioRhodopsin; 2D correlation spectroscopy; FT-IR spectroscopy;
D O I：
10.1366/0003702001948691
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
Two-dimensional Fourier transform infrared (2D FT-IR) spectroscopy was applied to study the slower states of the membrane protein bacterioRhodopsin (hR) photocycle, with bR adsorbed on a ZnSe attenuated total reflectance (ATR) crystal. The M and the N states of the bR photocycle could be discriminated, and 2D spectra revealed an N state absorption band at 1536 cm(-1) that is normally covered by the broader and bigger 1527 cm(-1) M state absorption band. 2D FT-IR absorbance changes are presented in a more convenient way for analysis than in traditional 2D FT-IR spectroscopy by using 2D-phase spectra and phase/amplitude calculations.
引用
收藏
页码：1659 / 1664
页数：6
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