Identification of novel piRNAs in bladder cancer

被引：120

作者：

Chu, Haiyan ^{[1
,2
,3
]}

Hui, Gaoyun ^{[1
,2
,3
]}

Yuan, Lin ^{[4
]}

Shi, Danni ^{[1
,2
,3
]}

Wang, Yubang ^{[5
]}

Du, Mulong ^{[1
,2
,3
]}

Zhong, Dongyan ^{[2
,3
]}

Ma, Lan ^{[1
,2
,3
]}

Tong, Na ^{[1
,2
,3
]}

Qin, Chao

Yin, Changjun ^{[6
]}

Zhang, Zhengdong ^{[1
,2
,3
]}

Wang, Meilin ^{[1
,2
,3
]}

机构：

[1] Nanjing Med Univ, Inst Toxicol, State Key Lab Reprod Med, Nanjing, Peoples R China

[2] Nanjing Med Univ, Prevent & Treatment Canc Ctr, Jiangsu Key Lab Canc Biomarkers, Dept Environm Gen, Nanjing, Peoples R China

[3] Nanjing Med Univ, Sch Publ Hlth, Minist Educ, Dept Genet Toxicol,Key Lab Modern Toxicol, Nanjing, Peoples R China

[4] Jiangsu Prov Hosp TCM, Dept Urol, Nanjing, Peoples R China

[5] Nanjing Med Univ, Sch Publ Hlth, Safety Assessment & Res Ctr Drug Pesticide & Vet, Nanjing, Peoples R China

[6] Nanjing Med Univ, Affiliated Hosp 1, Dept Urol, Nanjing, Peoples R China

来源：

CANCER LETTERS | 2015年 / 356卷 / 02期

基金：

中国国家自然科学基金;

关键词：

piRNA profiles; Bladder cancer; Biomarkers; HUMAN GASTRIC-CANCER; TUMOR-RELATED DEATH; SMALL RNAS; MUTATIONS RESULT; PIWI PROTEINS; OX40; LIGAND; HIWI GENE; CELLS; EXPRESSION; DROSOPHILA;

D O I：

10.1016/j.canlet.2014.10.004

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

PIWI-interacting RNAs (piRNAs) are a newly identified class of small non-coding RNAs that can play important roles in germline development and carcinogenesis. In this study, piRNA microarrays were used to investigate global piRNA expression in three bladder cancer tissues and their adjacent normal tissues. Using the 3' untranslated region (UTR) sequence complementarily method, we predicted the target gene of piRNA. Our results showed that the expression levels of 106 piRNAs were up-regulated and 91 were down-regulated in bladder cancer tissues, among which the fold-change of down-regulated piRNA DQ594040 associated with bladder cancer (piRABC) was the highest piRNA. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm piRABC expression in paired bladder cancer tissues and their adjacent normal tissues (n = 25). Over-expression of piRABC can inhibit bladder cancer cell proliferation, colony formation, and promote cell apoptosis (all P < 0.05). Luciferase reporter gene assays indicated that piRABC could increase the luciferase activity of TNFSF4. Western blotting analyses and ELISA assays also confirmed that the expression of TNFSF4 protein was up-regulated in control subjects compared with bladder cancer subjects. In conclusion, piRABC plays a crucial role in the development of bladder cancer. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：561 / 567

页数：7

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