Effect of belt electrode-skeletal muscle electrical stimulation on immobilization-induced muscle fibrosis

被引：14

作者：

Honda, Yuichiro ^{[1
]}

Tanaka, Natsumi ^{[2
,3
]}

Kajiwara, Yasuhiro ^{[2
,3
]}

Kondo, Yasutaka ^{[4
]}

Kataoka, Hideki ^{[3
,5
]}

Sakamoto, Junya ^{[1
]}

Akimoto, Ryuji ^{[6
]}

Nawata, Atsushi ^{[7
]}

Okita, Minoru ^{[1
,3
]}

机构：

[1] Nagasaki Univ, Inst Biomed Sci Hlth Sci, Nagasaki, Japan

[2] Nagasaki Univ Hosp, Dept Rehabil, Nagasaki, Japan

[3] Nagasaki Univ, Grad Sch Biomed Sci, Dept Phys Therapy Sci, Nagasaki, Japan

[4] Japanese Red Cross Nagasaki Genbaku Hosp, Dept Rehabil, Nagasaki, Japan

[5] Nagasaki Mem Hosp, Dept Rehabil, Nagasaki, Japan

[6] HOMER ION Co Ltd, Res & Dev Div, Shibuya Ku, Tokyo, Japan

[7] ALCARE Co Ltd, Med Engn Res Lab, Sumida Ku, Tokyo, Japan

来源：

PLOS ONE | 2021年 / 16卷 / 05期

关键词：

CELL-PROLIFERATION; ATROPHY; EXERCISE; DENERVATION; ULTRASOUND; HEART;

D O I：

10.1371/journal.pone.0244120

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purpose Macrophage accumulation in response to decreasing myonuclei may be the major mechanism underlying immobilization-induced muscle fibrosis in muscle contracture, an intervention strategy suppressing these lesions is necessary. Therefore, this research investigated the effect of belt electrode-skeletal muscle electrical stimulation (B-SES), a new electrical stimulation device, to the macrophage accumulation via myonuclei decrease in immobilization-induced muscle fibrosis. Materials and methods 18 Wistar male rats were divided into the control group, immobilization group (with plaster cast fixation to immobilize the soleus muscles in a shortened position for 2 weeks), and B-SES group (with muscle contractile exercise through B-SES during the immobilization period). B-SES stimulation was performed at a frequency of 50 Hz and an intensity of 4.7 mA, muscle contractile exercise by B-SES was applied to the lower limb muscles for 20 minutes/session (twice a day) for 2 weeks (6 times/week). The bilateral soleus muscles were used for histological, immunohistochemical, biochemical, and molecular biological analyses. Results The number of myonuclei was significantly higher in the B-SES group than in the immobilization group, and there was no significant difference between the B-SES and control groups. The cross-sectional area of type I and II myofibers in the immobilization and B-SES groups was significantly lower than that in the control group, and the cross-sectional area of type I myofibers in the B-SES group was higher than that in the immobilization group. However, Atrogin-1 and MuRF-1 mRNA expression in the immobilization and B-SES groups was significantly higher than those in the control group. Additionally, the number of macrophages, IL-1 beta, TGF-beta 1, and alpha -SMA mRNA expression, and hydroxyproline expression was significantly lower in the control and B-SES groups than those in the immobilization group. Conclusion This research surmised that muscle contractile exercise through B-SES prevented immobilization-induced muscle fibrosis, and this alteration suppressed the development of muscle contracture.

引用

页数：16