Sensitive electrochemical detection of amyloid beta peptide in human serum using an interdigitated chain-shaped electrode

被引：46
作者：
Le, Hien T. Ngoc [1 ]
Park, Jinsoo [2 ]
Chinnadayyala, Somasekhar R. [1 ]
Cho, Sungbo [1 ,2 ]
机构：
[1] Gachon Univ, Dept Elect Engn, 1342 Seongnamdaero, Seongnam Si 13120, Gyeonggi Do, South Korea
[2] Gachon Univ, GAIHST, Dept Hlth Sci & Technol, Incheon 21999, South Korea
来源：
BIOSENSORS & BIOELECTRONICS | 2019年 / 144卷
基金：
新加坡国家研究基金会;
关键词：
Interdigitated chain-shaped electrode; Amyloid-beta; 1-42; peptide; anti-a beta antibody; Self-assembled monolayer; Electrochemical biosensor; Electrochemical impedance spectroscopy; ALZHEIMERS-DISEASE; BIOSENSOR; DIAGNOSIS; IMMUNOSENSOR; BIOMARKERS; MILD;
D O I：
10.1016/j.bios.2019.111694
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this study, we developed a small size, low cost, highly sensitive electrochemical biosensor with a low limit of detection by immobilizing specific anti-amyloid-beta (a beta) antibody onto a self-assembled monolayer functionalized interdigitated chain-shaped electrode (anti-a beta/EDC-NHS/SAM/ICE). The anti-a beta/EDC-NHS/SAM/ICE specifically detects a beta 1-42 peptide (a peptide 1-42 amino acids long), which is one of main biomarkers of Alzheimer's disease in human serum (HS). Electrochemical impedance spectroscopy (EIS) was used to characterize the impedance change of the anti-a beta/EDC-NHS/SAM/ICE biosensor for a beta 1-42 detection, which provided a wide linear range of detection from 10(-3) -10(3) ng mL(-1), and a low limit of detection of a beta in HS (100 pg mL(-1)) much lower than the limit of detection of CSF a beta 1-42 (similar to 500 pg mL(-1)), and other biosensors. Therefore, the developed biosensor is sensitive enough to be used for the diagnosis of early stage Alzheimer's disease.
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页数：6
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