Plasmon-enhanced fluorescence correlation spectroscopy for super-localized detection of nanoscale subcellular dynamics

被引：9

作者：

Lee, Hongki ^{[1
]}

Rhee, Woo Joong ^{[2
]}

Moon, Gwiyeong ^{[1
]}

Im, Seongmin ^{[1
]}

Son, Taehwang ^{[1
,4
]}

Shin, Jeon-Soo ^{[2
,3
]}

Kim, Donghyun ^{[1
]}

机构：

[1] Yonsei Univ, Sch Elect & Elect Engn, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Dept Microbiol, Seoul 03722, South Korea

[3] Yonsei Univ, Coll Med, Project Med Sci BK21, Inst Immunol & Immunol Dis, Seoul 03722, South Korea

[4] Harvard Univ, MGH, Cambridge, MA 02138 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2021年 / 184卷

基金：

新加坡国家研究基金会;

关键词：

Fluorescence correlation spectroscopy; Plasmonics; Super-localization; Plasmonic nanodimer arrays; Nanogap; Lysosome; SINGLE-MOLECULE ANALYSIS; NEAR-FIELD; CELL; MICROSCOPY; TRANSMISSION; RESOLUTION; ORGANELLE; ARRAYS;

D O I：

10.1016/j.bios.2021.113219

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.

引用

页数：6

共 44 条

[1] Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections [J].