Interhelical spacing in liquid crystalline spermine and spermidine-DNA precipitates

被引:119
作者
Raspaud, E [1 ]
Durand, D
Livolant, F
机构
[1] Univ Paris 11, Phys Solides Lab, CNRS, UMR 8502, F-91405 Orsay, France
[2] Univ Paris 11, Inst Biochim & Biophys Mol & Cellulaire, CNRS, UMR 8619, F-91405 Orsay, France
关键词
D O I
10.1529/biophysj.104.040113
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The structure of polyamines-DNA precipitates was studied by x-ray diffraction. Precise measurements of the interhelix distance a(H) were obtained at different NaCl, polyamine, and DNA concentrations. Most of the results were obtained using spermine and few others using spermidine. The precipitates are liquid crystalline, either hexagonal and/or cholesteric, with an interhelical spacing that depends on the ionic concentrations and on the polyamine type. In our experimental conditions, the spacing varies from 28.15 to 33.4 Angstrom. This variation is interpreted in terms of different ionic components that are present inside the precipitates and that are thought to regulate the value of the cohesive energy of DNA. These results are discussed in relation to the biological processes requiring a closeness of double helices and to the role played by polyamine analogs in cancer therapy.
引用
收藏
页码:392 / 403
页数:12
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