Anti-biofilm, anti-hemolysis, and anti-virulence activities of black pepper, cananga, myrrh oils, and nerolidol against Staphylococcus aureus

被引:43
作者
Lee, Kayeon [1 ]
Lee, Jin-Hyung [1 ]
Kim, Soon-Il [2 ]
Cho, Moo Hwan [1 ]
Lee, Jintae [1 ]
机构
[1] Yeungnam Univ, Sch Chem Engn, Gyongsan 712749, South Korea
[2] Nareso Res Ctr, Suwon 441853, South Korea
基金
新加坡国家研究基金会;
关键词
Anti-virulence; Biofilm; Black pepper oil; Essential oil; Hemolysis; Staphylococcus aureus; ALPHA-HEMOLYSIN; CAENORHABDITIS-ELEGANS; EPIDERMIDIS BIOFILMS; ESCHERICHIA-COLI; PIPER-NIGRUM; BACTERIA; TOXIN; INFECTIONS; EXPRESSION; COMPONENTS;
D O I
10.1007/s00253-014-5903-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The long-term usage of antibiotics has resulted in the evolution of multidrug-resistant bacteria. Unlike antibiotics, anti-virulence approaches target bacterial virulence without affecting cell viability, which may be less prone to develop drug resistance. Staphylococcus aureus is a major human pathogen that produces diverse virulence factors, such as alpha-toxin, which is hemolytic. Also, biofilm formation of S. aureus is one of the mechanisms of its drug resistance. In this study, anti-biofilm screening of 83 essential oils showed that black pepper, cananga, and myrrh oils and their common constituent cis-nerolidol at 0.01 % markedly inhibited S. aureus biofilm formation. Furthermore, the three essential oils and cis-nerolidol at below 0.005 % almost abolished the hemolytic activity of S. aureus. Transcriptional analyses showed that black pepper oil down-regulated the expressions of the alpha-toxin gene (hla), the nuclease genes, and the regulatory genes. In addition, black pepper, cananga, and myrrh oils and cis-nerolidol attenuated S. aureus virulence in the nematode Caenorhabditis elegans. This study is one of the most extensive on anti-virulence screening using diverse essential oils and provides comprehensive data on the subject. This finding implies other beneficial effects of essential oils and suggests that black pepper, cananga, and myrrh oils have potential use as anti-virulence strategies against persistent S. aureus infections.
引用
收藏
页码:9447 / 9457
页数:11
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