A Two-Week Guided Inquiry Protein Separation and Detection Experiment for Undergraduate Biochemistry

被引：8

作者：

Carolan, James P. ^{[1
]}

Nolta, Kathleen V. ^{[2
]}

机构：

[1] Univ Michigan, Program Chem Biol, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CHEMICAL EDUCATION | 2016年 / 93卷 / 04期

关键词：

Upper-Division Undergraduate; Biochemistry; Laboratory Instruction; Hands-On Learning/Manipulatives; Inquiry-Based/Discovery Learning; Chromatography; Electrophoresis; Enzymes; Molecular Recognition; Proteins/Peptides; LABORATORY COURSE; PURIFICATION; INSTRUCTION; SEQUENCE; SCIENCE; PROJECT; BIOLOGY; YEAST; LONG;

D O I：

10.1021/acs.jchemed.5b00697

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A laboratory experiment for teaching protein separation and detection in an undergraduate biochemistry laboratory course is described. This experiment, performed in two, 4 h laboratory periods, incorporates guided inquiry principles to introduce students to the concepts behind and difficulties of protein purification. After using size-exclusion chromatography to separate a mixture of proteins, students utilize a colorimetric enzymatic assay and an immunoassay to determine the location of individual mixture components. Students proceed to determine the molecular weight of each protein using gel electrophoresis and generated mass spectrometric data. Completing this experiment provides students with an opportunity to expediently separate proteins while learning about protein characterization.

引用

页码：762 / 766

页数：5

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