Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro

被引:11
|
作者
Tsuruga, E [1 ]
Yajima, T [1 ]
Irie, K [1 ]
机构
[1] Hlth Sci Univ Hokkaido, Sch Dent, Dept Oral Anat, Ishikari, Hokkaido 0610293, Japan
来源
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2005年 / 37卷 / 01期
关键词
elastin deposition; elastogenesis; fibrillin; MAGP; tropoelastin;
D O I
10.1016/j.biocel.2004.06.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Microfibril-associated glycoprotein-1 (MAGP-1) and fibrillin-2, the major components of microfibrils, provide the likely template for tropoelastin deposition. In this study, we used the RNA interference (RNAi) technique to establish MAGP-1 and fibrillin-2 gene-specific knock-downs individually in elastin-producing cells (human gingival fibroblasts). We then examined the extracellular deposition of tropoelastin by western blotting. These two genes were specifically suppressed to <30% of the control level, and this was responsible for the diminution of tropoelastin deposition. An immunofluorescence study also confirmed that RNAi-mediated down-regulation of MAGP-1 or fibrillin-2 led to the loss of tropoelastin immunoreactivity. These results suggest that MAGP-1 and fibrillin-2 are, directly or indirectly, associated with the extracellular deposition of tropoelastin during elastic fiber formation in human gingival fibroblasts in vitro. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:120 / 129
页数:10
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