A simple method for the separation of Bacillus thuringiensis spores and crystals

被引：19
|
作者：
Mounsef, Jihane Rahbani [1 ,2 ,3 ]
Salameh, Dominique [3 ]
Awad, Mireille Kallassy [3 ]
Chamy, Laure [3 ]
Brandam, Cedric [1 ,2 ]
Lteif, Roger [3 ]
机构：
[1] Univ Toulouse, INPT, UPS, Lab Genie Chim, F-31030 Toulouse, France
[2] CNRS, Lab Genie Chim, F-31030 Toulouse, France
[3] Univ St Joseph Beyrouth, Beirut 11072050, Lebanon
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 107卷
关键词：
Bacillus thuringiensis; Crystals; Hexane; Purification; Spores; DELTA-ENDOTOXIN; PROTEIN; CELLS;
D O I：
10.1016/j.mimet.2014.10.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple new method, for separating Bacillus thuringiensis crystals from spores and cell debris, is described. The developed purification method uses hexane and low speed centrifugation and does not require any expensive material or reagents. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：147 / 149
页数：3
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