RP-HPLC METHOD FOR DETERMINATION OF OSELTAMIVIR PHOSPHATE IN CAPSULES AND SPIKED PLASMA

被引：12

作者：

Aydogmus, Zeynep ^{[1
]}

Caglar, Sena ^{[1
]}

Toker, Sidika ^{[1
]}

机构：

[1] Istanbul Univ, Fac Pharm, Dept Analyt Chem, TR-34116 Istanbul, Turkey

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 14期

关键词：

4-Chloro-7-nitrobenzofurazan; Drug analysis; HPLC; Oseltamivir phosphate; Plasma; INFLUENZA; NEURAMINIDASE; TAMIFLU(R); VALIDATION; CHROMATOGRAPHY; CARBOXYLATE; METABOLITE; INHIBITOR; URINE; ASSAY;

D O I：

10.1080/00032711003698721

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new, sensitive RP-HPLC method was developed for the determination of oseltamivir phosphate in capsules and plasma. The method was based on the reaction of the drug with 4-chloro-7-nitrobenzofurazan in borate buffer solution of pH 8.50. Isocratic chromatography was performed on a C(18) column with acetonitrile-10 mM nitric acid (pH 3, 60 + 40, v/v) as the mobile phase with fluorescence detection (lambda ex: 470 nm, lambda em: 541 nm). Mexiletine hydrochloride was used as an internal standard. Analytical parameters were evaluated. The calibration range was linear from 50.0-750.0 ng/ml. The mean percentage recovery in capsules and plasma were 99.95% and 95.42%, respectively.

引用

页码：2200 / 2209

页数：10

共 18 条

[1] Validated spectrofluorometric methods for determination of amlodipine besylate in tablets [J].

Abdel-Wadood, Hanaa A. ;

Mohamed, Niveen A. ;

Mahmoud, Ashraf A. .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2008, 70 (03) :564-570

[2] Simple and Sensitive Spectrofluorimetric Method for the Determination of Oseltamivir Phosphate in Capsules Through Derivatization with Fluorescamine [J].

Aydogmus, Zeynep .

JOURNAL OF FLUORESCENCE, 2009, 19 (04) :673-679

[3] Determination of oseltamivir carboxylic acid in human serum by solid phase extraction and high performance liquid chromatography with UV detection [J].

Bahrami, Gholamreza ;

Mohammadi, Bahareh ;

Kiani, Amir .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2008, 864 (1-2) :38-42

[4] High-performance liquid chromatographic determination of GS4071, a potent inhibitor of influenza neuraminidase, in plasma by precolumn fluorescence derivatization with naphthalenedialdehyde [J].

Eisenberg, EJ ;

Cundy, KC .

JOURNAL OF CHROMATOGRAPHY B, 1998, 716 (1-2) :267-273

[5]

Fuke Chiaki, 2008, Legal Medicine, V10, P83, DOI 10.1016/j.legalmed.2007.07.003

[6] Determination of oseltamivir quality by colorimetric and liquid chromatographic methods [J].

Green, Michael D. ;

Nettey, Henry ;

Wirtz, Robert A. .

EMERGING INFECTIOUS DISEASES, 2008, 14 (04) :552-556

[7] Influenza virus neuraminidase inhibitors [J].

Gubareva, LV ;

Kaiser, L ;

Hayden, FG .

LANCET, 2000, 355 (9206) :827-835

[8]

*HER MAJ STAT OFF, 1998, BRIT PHARM, P799

[9]

International Conference on Harmonisation-ICH, 1996, Q2(R1)

[10] Analysis of Oseltamivir in Tamiflu® Capsules Using Micellar Electrokinetic Chromatography [J].

Jabbaribar, Farnaz ;

Mortazavi, Afireza ;

Jalali-Milani, Reza ;

Jouyban, Abolghasem .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2008, 56 (12) :1639-1644

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