Frequency, virulence factors and antifungal susceptibility of Candida parapsilosis species complex isolated from patients with candidemia in the central region of Argentina

被引：11

作者：

Vigezzi, C. ^{[1
,2
]}

Icely, P. A. ^{[1
,2
]}

Dudiuk, C. ^{[3
,4
]}

Rodriguez, E. ^{[1
,2
]}

Miro, M. S. ^{[1
,2
]}

Castillo, G. D. V. ^{[5
]}

Azcurra, A. I. ^{[5
]}

Abiega, C. ^{[6
]}

Caeiro, J. P. ^{[6
]}

Riera, F. O. ^{[7
]}

Garcia-Effron, G. ^{[3
,4
]}

Sotomayor, C. E. ^{[1
,2
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Bioquim Clin, Lab Inmunidad Innata Patogenos Fung, Cordoba, Argentina

[2] Univ Nacl Cordoba, Fac Ciencias Quim, CIBICI CONICET, Ctr Invest Bioquim Clin & Inmunol, Cordoba, Argentina

[3] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Catedra Parasitol & Micol, Lab Micol & Diagnost Mol, Santa Fe, Argentina

[4] Consejo Nacl Invest Cient & Tecn, Santa Fe, Argentina

[5] Univ Nacl Cordoba, Fac Odontol, Dept Biol Bucal, Cordoba, Argentina

[6] Hosp Privado Univ Cordoba, Secc Enfermedades Infecciosas, Cordoba, Argentina

[7] Sanat Allende Cordoba, Dept Infectol, Cordoba, Argentina

来源：

JOURNAL DE MYCOLOGIE MEDICALE | 2019年 / 29卷 / 04期

关键词：

Candidemia; Candida albicans; C. parapsilosis cryptic species; Molecular typing; Virulence factors; Antifungal susceptibility; BLOOD-STREAM INFECTIONS; IDENTIFICATION; ORTHOPSILOSIS; ECHINOCANDIN; EPIDEMIOLOGY; SURVEILLANCE; FLUCONAZOLE; RESISTANCE; IMMUNE;

D O I：

10.1016/j.mycmed.2019.100907

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Purpose. - Our objectives were to report species distribution and survival of patients with candidemia in Argentina's central region and to establish the prevalence of C.parapsilosis sensu lato species, their virulence factors and their antifungal susceptibility profiles. Methods. - Yeasts isolated from bloodstream infections in Cordoba (Argentina) (n = 35) were molecularly identified. The production of lipase and acid aspartic protease (Sap), the adhesion capacity, and the isolates' ability to form biofilm were evaluated. The in vitro activity of 7 antifungal drugs was evaluated (CLSldocument M27-4thed). Results. - C. albicans was the most prevalent species (48.57%) followed by C. parapsilosis sensu lato (28.57%). The 30-day survival rate for C. albicans candidemia was slightly lower than non-albicans blood infections (50.00% vs. 57.90%). C. parapsilosis sensu stricto and C. orthopsilosis account for 60% and 40% of the cryptic species. Sap production and biofilm formation capacity were higher in C. parapsilosis sensu strico than in C. orthopsilosis. All the strains were susceptible to caspofungin (CAS), anidulafungin (AFG), amphotericin B (AMB), posaconazole (POS) and voriconazole (VRC). Azoles were the most potent agent against C. parapsilosis sensu lato followed by echinocandins and AMB. There were no differences between MICs for fluconazole, VRC, POS and AMB. Contrarily, C parapsilosis sensu stricto strains showed lower MIC than C. orthopsilopsis isolates for itraconazole and higher MIC values for echinocandins (P < 0.01). Conclusions. - We report a high frequency of isolation of C. orthopsilosis in candidemia patients of central region. Data on the prevalence, virulence capability and antifungal susceptibility of C. parapsilosis complex provide new epidemiological information about these cryptic species in Argentina. (C) 2019 Elsevier Masson SAS. All rights reserved.

引用

页码：285 / 291

页数：7

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