Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus

被引：45
作者：
Kuroda, Makoto
Nagasaki, Sanae
Ito, Ryuta
Ohta, Toshiko
机构：
[1] Natl Inst Infect Dis, Ctr Pathogen Genom, Lab Bacterial Genom, Shinjuku Ku, Tokyo 1628640, Japan
[2] Univ Tsukuba, Grad Sch Comprehens Human Sci, Dept Microbiol, Tsukuba, Ibaraki 305, Japan
来源：
FEMS MICROBIOLOGY LETTERS | 2007年 / 273卷 / 01期
关键词：
Staphylococcus aureus; farnesol; lipase; inhibitor; MOLECULAR CHARACTERIZATION; PURIFICATION; AGENTS; SUSCEPTIBILITY; BIOSYNTHESIS; SPECIFICITY; COLI;
D O I：
10.1111/j.1574-6968.2007.00772.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L-1) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophenyl palmitate (pNPP) revealed that the inhibitory action of farnesol appears to be the result of the inhibition of lipase activity rather than of its secretion into the culture medium. The inhibition was observed in all the tested 8 methicillin-susceptible S. aureus and 31 methicillin-resistant S. aureus clinical isolates. Using homogeneous lipase purified by hydrophobic interaction chromatography, it was revealed that farnesol could competitively inhibit the lipase activity against the substrate pNPP.
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收藏
页码：28 / 34
页数：7
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