Purification and characterization of an extracellular α-L-arabinofuranosidase from Fusarium oxysporum

被引：7

作者：

Christakopoulos, P ^{[1
]}

Katapodis, P ^{[1
]}

Hatzinikolaou, DG ^{[1
]}

Kekos, D ^{[1
]}

Macris, BJ ^{[1
]}

机构：

[1] Natl Tech Univ Athens, Sector 4, Dept Chem Engn, Biotechnol Lab, Zografou 15780, Attica, Greece

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2000年 / 87卷 / 02期

关键词：

Fusarium oxysporum; alpha-L-arabinofuranosidase; purification; characterization; synergism;

D O I：

10.1385/ABAB:87:2:127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An alpha-L-arabinofuranosidase from Fusarium oxysporum Fusarium oxysporum F3 was purified to homogeneity by a two-step ion exchange intercalated by a gel filtration chromatography. The enzyme had a molecular mass of 66 kDa and was optimally active at pH 6.0 and 60 degrees C. It hydrolyzed aryl alpha-L-arabinofuranosides and cleaved arabinosyl side chains from arabinoxylan and arabinan. There was a marked synergistic effect between the alpha-L-arabinofuranosidase and an endo-(1-->4)-beta-D-xylanase produced by F. oxysporum in the extensive hydrolysis of arabinoxylan.

引用

页码：127 / 133

页数：7

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