Fine-Tuning Next-Generation Genome Editing Tools

被引：43

作者：

Kanchiswamy, Chidananda Nagamangala ^{[1
]}

Maffei, Massimo ^{[2
]}

Malnoy, Mickael ^{[1
]}

Velasco, Riccardo ^{[1
]}

Kim, Jin-Soo ^{[3
,4
]}

机构：

[1] FEM, Genom & Biol Fruit Crop Dept, Res & Innovat Ctr, Via Mach 1, I-38010 San Michele All Adige, TN, Italy

[2] Univ Turin, Innovat Ctr, Dept Life Sci & Syst Biol, Via Quarello 15-A, I-10135 Turin, Italy

[3] Inst for Basic Sci Korea, Ctr Genome Engn, Gwanak Ro 1, Seoul 151747, South Korea

[4] Seoul Natl Univ, Dept Chem, Gwanak Ro 1, Seoul 151747, South Korea

来源：

TRENDS IN BIOTECHNOLOGY | 2016年 / 34卷 / 07期

关键词：

OFF-TARGET CLEAVAGE; RNA-GUIDED ENDONUCLEASES; ZINC-FINGER NUCLEASES; CRISPR-CAS NUCLEASES; HUMAN-CELLS; WIDE ANALYSIS; DNA CLEAVAGE; DIGENOME-SEQ; SPECIFICITY; GENE;

D O I：

10.1016/j.tibtech.2016.03.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The availability of genome sequences of numerous organisms and the revolution brought about by genome editing tools (e.g., ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has provided a breakthrough in introducing targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. However, the wider application of these tools in biology, agriculture, medicine, and biotechnology is limited by off-target mutation effects. In this review, we compare available methods for detecting, measuring, and analyzing off-target mutations. Furthermore, we particularly focus on CRISPR/Cas9 regarding various methods, tweaks, and software tools available to nullify off-target effects.

引用

页码：562 / 574

页数：13

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