Effect of testosterone on n-methyl-d, 1-aspartate-induced growth hormone secretion in male swine

Previous research from our laboratory demonstrated that n-methyl-d, 1-aspartate (NMA), a potent agonist of glutamate, increased growth hormone (GH) secretion in barrows and boars. To determine if testosterone modulates NMA-induced GH secretion, Poland China X Yorkshire swine were challenged with NMA in a model that compared GH responses in boars with those of barrows or barrows treated with testosterone propionate (TP). Boars and barrows weighing 112.6 +/- 1.4 kg (mean +/- SE) were fitted with indwelling jugular vein catheters. Barrows (n = 16) were given i.m. injections of TP (25 mg in corn oil) twice daily from d 0 to d 6. Boars (n = 16) and control barrows (n = 15) received twice daily injections of corn oil. On d 6, blood was sampled every 15 min for 4 h. Two h after sampling began, all animals received an i.v. injection of NMA at a dose of 2.5 mg/kg body weight. Mean testosterone concentrations (ng/ml serum) were similar (P >.1) for boars (8.1 +/- 0.8) and barrows receiving TP (7.3 +/- 0.3), but were greater in both cases (P < .05) than for barrows receiving corn oil (.2 +/- .01). Prior to NMA injections, mean GH concentrations were similar (P > .1) among groups and averaged 2.7 +/- .2 ng/ml serum across treatments. Serum concentrations of GH after NMA increased (P < .05) similarly among groups and averaged 6.3 +/- 0.3 ng/ml across treatments during the 2-h period after injection. These results were not supportive of a role for testosterone as a modulator of NMA-induced GH secretion in male swine. (C) 2000 Elsevier Science Inc. All rights reserved.