COMPARISON OF ELECTROPHORETIC PROTEIN PROFILES OF Brassica rapa SUB-SPECIES BROWN SARSON THROUGH SDS-PAGE METHOD

Estimation of protein based variability among different Brassica sub-species is important for crop improvement. In present study total seed protein based variation among brown sub-specie of B. rapa was studied. Twenty different brown types' genotypes were analysed through Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) method. The small, medium and large sizes proteins were noted. A total of 12 bands were obtained in which 10 (83.33%) are highly polymorphic while the rest two (15.38%) are monomorphic. The protein size base polymorphism was divided into different groups on the basis of molecular weight that ranges from similar to 10 kDa to similar to 180 kDa. The data of variable proteins were analysed through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method, which clustered all genotypes into four main cluster groups. The cluster I and II, III and IV consisted 3, 3, 6 and 10 genotypes respectively. The similarity coefficient values (40 to 96%) were calculated among different genotypes. The maximum similarity coefficient (96%) was recorded among genotypes Br-607, Br-560, and between Br-589 and Br-607 respectively. Our study showed maximum protein based diversity among brown sub-species of B. rapa which may serve as model to search protein based variation in other important plants sub-species.