Structural characterization of polysaccharide from Centipeda minima and its hypoglycemic activity through alleviating insulin resistance of hepatic HepG2 cells

被引：36
作者：
Chen, Juncheng [1 ]
Li, Lin [1 ,3 ]
Zhang, Xia [1 ]
Wan, Liting [1 ]
Zheng, Qingsong [1 ]
Xu, Dan [1 ]
Li, Yuting [3 ]
Liang, Yi [4 ]
Chen, Meisi [5 ]
Li, Bing [1 ]
Chen, Zhiyi [2 ]
机构：
[1] South China Univ Technol, Engn Res Ctr Starch & Plant Prot Deep Proc, Sch Food Sci & Engn,Minist Educ, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Peoples R China
[2] Guangdong Acad Agr Sci, Sericultural & Agrifood Res Inst, Guangdong Key Lab Agr Prod Proc, Minist Agr & Rural Affairs,Key Lab Funct Foods, Guangzhou 510610, Peoples R China
[3] Dongguan Univ Technol, Sch Chem Engn & Energy Technol, Dongguan 523808, Peoples R China
[4] Guangdong Chongqing Font Biochem Sci & Tech Co, Maoming 525427, Peoples R China
[5] Jiangmen Food Inspect Inst, Jiangmen 529000, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2021年 / 82卷
基金：
国家重点研发计划;
关键词：
Polysaccharide; Characterization; Hypoglycemic activity; Insulin-resistance HepG2 cells; ALPHA-GLUCOSIDASE INHIBITORS; ANTIOXIDANT ACTIVITY; ACIDIC POLYSACCHARIDE; LYCIUM-BARBARUM; PURIFICATION;
D O I：
10.1016/j.jff.2021.104478
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A novel polysaccharide (CMP-2B) was purified from Centipeda minima by anion-exchange and gel-permeation chromatography. Structural characterization revealed that CMP-2B was a homogeneous heteropolysaccharide with an average molecular weight of 113.193 KDa. CMP-2B was made up of mannose, galacturonic acid, galactose and arabinose with a molar ratio of 0.27:0.12:0.42:0.17. The backbone chain of CMP-2B consisted of 3,6)-alpha-D-Manp-(1 and 4,6)-beta-D-Galp-(1, and the branches were comprised of 4)-alpha-D-GalpA-(1, 6)-beta-D-Galp-(1, T alpha-L-Araf-(1 and 3)-alpha-L-Araf -(1. Besides, the results of antioxidant assays revealed that CMP-2B possessed signifcant free radical scavenging ability. Moreover, the results of hypoglycemic assays showed that CMP-2B displayed alpha-glucosidase inhibitory activities, and could significantly improve glucose consumption, glycogen synthesis and the activity of pyruvate kinase of insulin-resistance HepG2 cells. Overall, these results suggested that CMP-2B could be a new source for functional foods.
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页数：11
相关论文
共 62 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   NMR spectroscopy in the study of carbohydrates: Characterizing the structural complexity [J].
Bubb, WA .
CONCEPTS IN MAGNETIC RESONANCE PART A, 2003, 19A (01) :1-19
[3]   Structural characterization, antioxidant and hepatoprotective activities of polysaccharides from Sophorae tonkinensis Radix [J].
Cai, Liangliang ;
Zou, Shanshan ;
Liang, Dengpan ;
Luan, Libiao .
CARBOHYDRATE POLYMERS, 2018, 184 :354-365
[4]   Physicochemical characterization, potential antioxidant and hypoglycemic activity of polysaccharide from Sargassum pallidum [J].
Cao, Changliang ;
Li, Chao ;
Chen, Qing ;
Huang, Qiang ;
Mondragon Perez, Manuel Everardo ;
Fu, Xiong .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 139 :1009-1017
[5]   Structure and in vitro hypoglycemic activity of a homogenous polysaccharide purified from Sargassum pallidum [J].
Cao, Changliang ;
Zhang, Bin ;
Li, Chao ;
Huang, Qiang ;
Fu, Xiong ;
Liu, Rui Hai .
FOOD & FUNCTION, 2019, 10 (05) :2828-2838
[6]   Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus [J].
Chen, Juncheng ;
Zhang, Xia ;
Huo, Da ;
Cao, Changliang ;
Li, Yuting ;
Liang, Yi ;
Li, Bing ;
Li, Lin .
CARBOHYDRATE POLYMERS, 2019, 215 :307-315
[7]  
Chen JC, 2018, FOOD FUNCT, V9, P6338, DOI [10.1039/c8fo01117f, 10.1039/C8FO01117F]
[8]   Structural characterization and α-glucosidase inhibitory activity of polysaccharides extracted from Chinese traditional medicine Huidouba [J].
Chen, Juncheng ;
Li, Lin ;
Zhou, Xin ;
Li, Bing ;
Zhang, Xia ;
Hui, Rong .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 117 :815-819
[9]   Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp [J].
Cui, Jie ;
Gu, Xin ;
Wang, Fengjun ;
Ouyang, Jie ;
Wang, Jianzhong .
CARBOHYDRATE POLYMERS, 2015, 121 :309-314
[10]   Antidiabetic activity of levan polysaccharide in alloxan-induced diabetic rats [J].
Dahech, Imen ;
Belghith, Karima Srih ;
Hamden, Khaled ;
Feki, Abdelfattah ;
Belghith, Hafedh ;
Mejdoub, Hafedh .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 49 (04) :742-746
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