Time course of epiphyseal growth plate fusion in rat tibiae

被引:77
作者
Martin, EA
Ritman, EL
Turner, RT
机构
[1] Mayo Clin, Dept Physiol & Biophys, Rochester, MN 55905 USA
[2] Mayo Clin, Mayo Grad Sch, Rochester, MN 55905 USA
[3] Mayo Clin, Dept Orthoped, Rochester, MN 55905 USA
[4] Mayo Clin, Dept Biochem, Rochester, MN 55905 USA
[5] Mayo Clin, Dept Biol Mol, Rochester, MN 55905 USA
关键词
microcomputed tomography; cancellous bone; epiphyseal growth plate; rat tibia; aging; 3-D microarchitecture; TRABECULAR BONE; COMPUTED-TOMOGRAPHY; CANCELLOUS BONE; IN-VIVO; ARCHITECTURE; MORPHOLOGY; OSTEOPENIA; AGE;
D O I
10.1016/S8756-3282(02)00983-3
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Although the rat is the most common animal model used in studying osteoporosis, it is often used inappropriately. Osteoporosis is a disease that most commonly occurs in humans long after growth plate fusion with the associated cessation of longitudinal bone growth, but there has been a question as to when or to what extent the rat growth plate fuses. To investigate this question, we used microcomputed X-ray tomography, at voxel resolutions ranging from (5.7 mum)(3) to (11 mum)(3), to image the proximal epiphyseal growth plates of both male (n = 19) and female (n = 15) rat tibiae, ranging in age from 2 to 25 months. The three-dimensional images were used to evaluate fusion of the epiphyseal growth plate by quantitating the amount of cancellous bone that has bridged across the growth plate. The results suggest that the time course of fusion of the epiphyseal growth plate follows a sigmoidal pattern, with 10% of the maximum number of bridges having formed by 3.9 months in the male tibiae and 5.8 months in the female tibiae, 50% of the maximum number of bridges having formed by 5.6 months in the male tibiae and 5.9 months in the female tibiae, and 90% of the total maximum of bridges have formed by 7.4 months for the males and 6.5 months for the females. The total volume of bridges per tibia at the age at which the maximum number of bridges per tibia has first formed is 0.99 mm(3)/tibia for the males and 0.40 mm(3)/tibia for the females. After the maximum number of bridges (-290 for females, -360 for males) have formed the total volume of bridges per tibia continues to increase for an additional 7.0 months in the males and 17.0 months for the females until they reach maximum values (-1.5 mm(3) /tibia for the males and -2.2 mm(3)/tibia for the females). (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:261 / 267
页数:7
相关论文
共 34 条
[1]  
Arnold J., 1980, Metab Bone Dis Rel Res, V2, P297
[2]   Prepubertal oophorectomy limits the accumulation of cancellous bone in the femur of growing rats with long-term effects on metaphyseal bone architecture [J].
Baldock, PAJ ;
Morris, HA ;
Moore, RJ ;
Need, AG ;
Durbridge, TC .
CALCIFIED TISSUE INTERNATIONAL, 1998, 62 (03) :244-249
[3]   MEASUREMENT OF ANISOTROPIC VERTEBRAL TRABECULAR BONE LOSS DURING AGING BY QUANTITATIVE IMAGE-ANALYSIS [J].
BERGOT, C ;
LAVALJEANTET, AM ;
PRETEUX, F ;
MEUNIER, A .
CALCIFIED TISSUE INTERNATIONAL, 1988, 43 (03) :143-149
[4]  
BRIGHTON CT, 1987, RHEUM DIS CLIN N AM, V13, P75
[5]  
Erben RG, 1996, ANAT REC, V246, P39, DOI 10.1002/(SICI)1097-0185(199609)246:1<39::AID-AR5>3.0.CO
[6]  
2-A
[7]   TRABECULAR BONE-RESORPTION DEPTH DECREASES WITH AGE - DIFFERENCES BETWEEN NORMAL MALES AND FEMALES [J].
ERIKSEN, EF ;
MOSEKILDE, L ;
MELSEN, F .
BONE, 1985, 6 (03) :141-146
[8]   THE DIRECT EXAMINATION OF 3-DIMENSIONAL BONE ARCHITECTURE INVITRO BY COMPUTED-TOMOGRAPHY [J].
FELDKAMP, LA ;
GOLDSTEIN, SA ;
PARFITT, AM ;
JESION, G ;
KLEEREKOPER, M .
JOURNAL OF BONE AND MINERAL RESEARCH, 1989, 4 (01) :3-11
[9]   MEASUREMENT AND SIGNIFICANCE OF 3-DIMENSIONAL ARCHITECTURE TO THE MECHANICAL INTEGRITY OF TRABECULAR BONE [J].
GOLDSTEIN, SA ;
GOULET, R ;
MCCUBBREY, D ;
GRYNPAS ;
MONIERFAUGERE ;
BIEWENER .
CALCIFIED TISSUE INTERNATIONAL, 1993, 53 :S127-S133
[10]   In vivo assessment of trabecular bone structure at the distal radius from high-resolution computed tomography images [J].
Gordon, CL ;
Webber, CE ;
Adachi, JD ;
Christoforou, N .
PHYSICS IN MEDICINE AND BIOLOGY, 1996, 41 (03) :495-508