The effects of heavy long-term exercise on ventricular myocyte shortening and intracellular Ca2+ in streptozotocin-induced diabetic rat

Objective: This study investigated whether exercise training, initiated at the onset of diabetes, could preserve the contractile properties of ventricular myocytes. Research Design and Methods: The effects of a heavy exercise training program on shortening and intracellular Ca2+ in unloaded ventricular myocytes from streptozotocin (STZ)-induced diabetic rats were examined. Animals were divided into four groups: control sedentary (CS), diabetic sedentary (DS), control heavy exercise (CUE), and diabetic heavy exercise (DHE). Exercise protocol: 5x60 min/week, 18 m/min, 5% gradient. Exercise training began 1 week after STZ treatment and continued for 12-23 (mean 17.5) weeks. Results: Diabetes induced prolongation of time-to-peak (TPK) shortening (124 +/- 2 ms in DS compared to 97 +/- 2 ms in CS rats), which was further increased by exercise (133 +/- 3 ms in DHE and 112 +/- 2 ins in CHE myocytes). Diabetes had no significant effects on time-to-half (THALF) relaxation of shortening (61 +/- 2 ms in DS compared to 56 +/- 2 ms in CS myocytes). Exercise induced significant prolongation of THALF in control (66 +/- 3 ms) but not in diabetic (69 +/- 3 ms) myocytes. Diabetes, though not exercise, significantly prolonged TPK (76 +/- 3 ms in DS compared to 64 +/- 2 ms in CS) and THALF recovery (160 +/- 5 ms in DS compared to 118 +/- 4 ms in CS) of the Ca2+ transient. Neither diabetes nor exercise had significant effects on the amplitude of myocyte shortening and the Ca2+ transient. Conclusions: Heavy long-term exercise alters the dynamics but not the amplitude of unloaded myocyte contraction in the STZ-induced diabetic rat. (C) 2010 Elsevier Inc. All rights reserved.