A Novel Pipeline for V(D)J Junction Identification using RNA-Seq Paired-end Reads

被引:0
作者
Paciello, Giulia [1 ]
Ficarra, Elisa [1 ]
Zamo, Alberto [2 ]
Pighi, Chiara [2 ]
Foti, Carmelo [1 ]
Abate, Francesco [1 ,3 ]
Macii, Enrico [1 ]
Acquaviva, Andrea [1 ]
机构
[1] Politecn Torino, Dept Comp Engn, Turin, Italy
[2] Univ Verona, Dept Pathol & Diagnost, I-37100 Verona, Italy
[3] Columbia Univ, Coll Phys & Surg, Dept Biomed Informat Ctr Computat Biol & Bioinf, New York, NY 10027 USA
来源
BIOINFORMATICS 2013: PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON BIOINFORMATICS MODELS, METHODS AND ALGORITHMS | 2013年
关键词
Antibody; V(D)J Junction; RNA-Seq Data;
D O I
暂无
中图分类号
R-058 [];
学科分类号
摘要
Immunoglobulin heavy and light chains are assembled respectively from germline V, D, J and V, J segments within a process called V(D) J recombination involving the development of T and B lymphocytes. The discovery that abnormal antibodies are often related to a wide range of pathologies conducted during the last years to many studies inherent the immunoglobulin features. In particular the identification of the functional V(D) J sequence of an antibody is considered fundamental since it could allow to understand the link between a particular disease and a specific recombination in a certain tissue and to promote the engineering of therapeutic antibodies. Objective of the implemented pipeline consists in the identification of the so called 'main clone' that characterizes a neoplastic tissue using paired-end RNA-Sequencing (RNA-Seq) reads.
引用
收藏
页码:185 / 189
页数:5
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