Further studies on the pharmacological profile of the neuropeptide S receptor antagonist SHA 68

被引：53

作者：

Ruzza, Chiara ^{[1
,2
,3
]}

Rizzi, Anna ^{[1
,2
,3
]}

Trapella, Claudio ^{[4
,5
]}

Pela', Michela ^{[4
,5
]}

Camarda, Valeria ^{[1
,2
,3
,6
]}

Ruggieri, Valentina ^{[6
]}

Filaferro, Monica

Cifani, Carlo ^{[7
]}

Reinscheid, Rainer K. ^{[8
]}

Vitale, Giovanni ^{[6
]}

Ciccocioppo, Roberto ^{[7
]}

Salvadori, Severo ^{[4
,5
]}

Guerrini, Remo ^{[4
,5
]}

Calo', Girolamo ^{[1
,2
,3
]}

机构：

[1] Univ Ferrara, Dept Expt & Clin Med, Pharmacol Sect, I-44100 Ferrara, Italy

[2] Univ Ferrara, Ctr Neurosci, I-44100 Ferrara, Italy

[3] Univ Ferrara, Natl Inst Neurosci, I-44100 Ferrara, Italy

[4] Univ Ferrara, Dept Pharmaceut Sci, I-44100 Ferrara, Italy

[5] Univ Ferrara, Ctr Biotechnol, I-44100 Ferrara, Italy

[6] Univ Modena & Reggio Emilia, Dept Biomed Sci, Pharmacol Sect, I-41100 Modena, Italy

[7] Univ Camerino, Dept Pharmacol Sci & Expt Med, I-62032 Camerino, Italy

[8] Univ Calif Irvine, Dept Pharmaceut Sci, Irvine, CA 92697 USA

来源：

PEPTIDES | 2010年 / 31卷 / 05期

关键词：

Neuropeptide S; NPS receptor; SHA; 68; Calcium assay; In vivo studies; ELEVATED PLUS-MAZE; FOOD-INTAKE; LOCOMOTOR-ACTIVITY; MICE; IDENTIFICATION; EXPRESSION; RATS; HYPOTHALAMUS; ACTIVATION; AMYGDALA;

D O I：

10.1016/j.peptides.2010.02.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neuropeptide S (NPS) regulates various biological functions by selectively activating the NPS receptor (NPSR). Previous studies demonstrated that the non-peptide molecule SHA 68 acts as a selective NPSR antagonist. In the present study the pharmacological profile of SHA 68 has been further investigated in vitro and in vivo. In cells expressing the mouse NPSR SHA 68 was inactive per se up to 10 mu M while it antagonized NPS-stimulated calcium mobilization in a competitive manner showing a pA(2) value of 8.06. In the 10-50 mg/kg range of doses, SHA 68 counteracted the stimulant effects elicited by NPS, but not those of caffeine, in mouse locomotor activity experiments. In the mouse righting reflex assay SHA 68 fully prevented the arousal-promoting action of the peptide. The anxiolytic-like effects of NPS were slightly reduced by SHA 68 in the mouse open field, fully prevented in the rat elevated plus maze and partially antagonized in the rat defensive burying paradigm. Finally, SHA 68 was found poorly active in antagonizing the NPS inhibitory effect on palatable food intake in rats. In all assays SHA 68 did not produce any effect per se. In conclusion, the present study demonstrated that SHA 68 behaves as a selective NPSR antagonist that can be used to characterize the in vivo actions of NPS. However the usefulness of this research tool is limited by its poor pharmacokinetic properties. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：915 / 925

页数：11

共 45 条

[1] Expression and function of NPSR1/GPRA in the lung before and after induction of asthma-like disease [J].