Short-term lime application impacts microbial community composition and potential function in an acid black soil

被引:48
作者
Li, Sen [1 ,2 ]
Liu, Junjie [1 ]
Yao, Qin [1 ]
Yu, Zhenhua [1 ]
Li, Yansheng [1 ]
Jin, Jian [1 ]
Liu, Xiaobing [1 ]
Wang, Guanghua [1 ]
机构
[1] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
中国国家自然科学基金;
关键词
Bacterial community; Fungal community; Pot experiment; Soybean · MiSeq sequencing; ARBUSCULAR MYCORRHIZAL FUNGI; CHEMICAL-PROPERTIES; PH; ACIDIFICATION; BACTERIAL; DIVERSITY; CARBON; YIELD; NITROGEN; TILLAGE;
D O I
10.1007/s11104-021-04913-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Background and aims Soil acidification is a natural process that can be accelerated by intensive nitrogen fertilization. Lime application is a typical agricultural practice to enhance soil pH and increase nutrient availability for crop production. Our study aims to reveal how liming altered soil microbial community composition and potential function in the bulk and rhizosphere soils of soybean growing in an acid black soil. Methods A short-term soybean pot experiment was conducted in an acid black soil with the amendment of five different dosages of lime. Soybean plants were harvested and soil samples were collected at the initial flowering stage. Plant biomass, shoot height and root length, as well as soil chemical properties and total microbial activities of bulk soils were measured. The abundance and composition of microbial communities in bulk and rhizosphere soils were determined using qPCR and Illumina MiSeq sequencing, respectively. Results Liming significantly increased soybean growth and soil microbial activities, and altered soil properties such as soil pH, available phosphorus (AP), ammonium nitrogen (NH4+-N), nitrate nitrogen (NO3--N). Lime addition increased soil bacterial abundance and decreased fungal abundance in the bulk soils, but had no effect on microbial abundance in the rhizosphere soils as well as alpha-diversity of soil microbial community. Microbial community structures in bulk and rhizosphere soils were significantly varied with lime amendment that were related to soil chemical properties, of which soil pH was detected as the most important soil factors. In addition, liming significantly increased the potential functions of amino acid, cofactors and vitamins mechanisms of bacterial communities and the guild abundance of AM fungi. Conclusions Lime application altered soil properties, increased soil microbial activities, and changed soil microbial compositions and potential functions, which eventually resulted in the improvement of soybean plant growth.
引用
收藏
页码:35 / 50
页数:16
相关论文
共 72 条
[1]   Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils [J].
Adam, G ;
Duncan, H .
SOIL BIOLOGY & BIOCHEMISTRY, 2001, 33 (7-8) :943-951
[2]   Molecular identification of potential pathogens in water and air of a hospital therapy pool [J].
Angenent, LT ;
Kelley, ST ;
St Amand, A ;
Pace, NR ;
Hernandez, MT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (13) :4860-4865
[3]   Improving barley yield on an acidic Boralf with crop rotation, lime, and zero tillage [J].
Arshad, MA ;
Franzluebbers, AJ ;
Gill, KS .
SOIL & TILLAGE RESEARCH, 1999, 50 (01) :47-53
[4]   Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth [J].
Artursson, V ;
Finlay, RD ;
Jansson, JK .
ENVIRONMENTAL MICROBIOLOGY, 2006, 8 (01) :1-10
[5]   Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data [J].
Asshauer, Kathrin P. ;
Wemheuer, Bernd ;
Daniel, Rolf ;
Meinicke, Peter .
BIOINFORMATICS, 2015, 31 (17) :2882-2884
[6]   Changing pH shifts the microbial sourceas well as the magnitude of N2O emission from soil [J].
Baggs, Elizabeth M. ;
Smales, Claire L. ;
Bateman, Emma J. .
BIOLOGY AND FERTILITY OF SOILS, 2010, 46 (08) :793-805
[7]   Soil acidification in continuously cropped tobacco alters bacterial community structure and diversity via the accumulation of phenolic acids [J].
Bai, Yuxiang ;
Wang, Ge ;
Cheng, Yadong ;
Shi, Puyou ;
Yang, Chengcui ;
Yang, Huanwen ;
Xu, Zhaoli .
SCIENTIFIC REPORTS, 2019, 9 (1)
[8]   Belowground biodiversity and ecosystem functioning [J].
Bardgett, Richard D. ;
van der Putten, Wim H. .
NATURE, 2014, 515 (7528) :505-511
[9]   Crop Residue Removal Impacts on Soil Productivity and Environmental Quality [J].
Blanco-Canqui, Humberto ;
Lal, R. .
CRITICAL REVIEWS IN PLANT SCIENCES, 2009, 28 (03) :139-163
[10]   A global analysis of acidification and eutrophication of terrestrial ecosystems [J].
Bouwman, AF ;
Van Vuuren, DP ;
Derwent, RG ;
Posch, M .
WATER AIR AND SOIL POLLUTION, 2002, 141 (1-4) :349-382