Fluorescent silver nanoclusters as probes for selective recognition of DNA CGG trinucleotide repeat

被引：15

作者：

Liu, Hua ^{[1
]}

Wang, Ying ^{[1
]}

Zhang, Lihua ^{[1
]}

Shao, Yong ^{[1
]}

Zheng, Bin ^{[2
]}

机构：

[1] Zhejiang Normal Univ, Inst Phys Chem, Jinhua 321004, Zhejiang, Peoples R China

[2] Zhejiang Prov Peoples Hosp, Dept Otorhinolaryngol, Hangzhou 310014, Zhejiang, Peoples R China

来源：

MATERIALS LETTERS | 2015年 / 139卷

基金：

中国国家自然科学基金;

关键词：

Trinucleotide repeat; Nucleic acid; Silver nanoclusters; Luminescence; Selective recognition; Sensors; EMISSION;

D O I：

10.1016/j.matlet.2014.10.108

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Silver nanoclusters (AgNCs) are an emerging set of fluorescent materials that are advantageous over semiconductor quantum dots and organic fluorophores. Herein, fluorescent AgNCs were used as potential probes for selective recognition of DNA (CGG) trinucleotide repeat that is more related to diseases. In comparison with (CCG), (CAG), and (CTG) trinucleotide repeats, (CGG) repeat is much more efficient to produce fluorescent AgNCs. Thus, AgNCs can serve as useful fluorophores for high selective recognition of (CGG) repeat. Additionally, we used molecular crowding condition to further sensitize the (CGG)-grown AgNCs' fluorescence without sacrificing the high sequence selectivity. Unlike the usually used organic fluorophores, AgNCs can be in situ created at the moment of manipulation and the fluorescence is not quenched by guanine in (CGG) repeat, the most easily oxidizable base in nucleic acids. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：265 / 267

页数：3

共 20 条

[1]

[Anonymous], 2012, BIOORG MED CHEM LETT

[2]

[Anonymous], 2011, CHEM COMMUN

[3] Validation of a Screening Tool for the Rapid and Reliable Detection of CGG Trinucleotide Repeat Expansions in FMR1 [J].

Basehore, Monica J. ;

Marlowe, Natalia M. ;

Jones, Julie R. ;

Behlendorf, Deborah E. ;

Laver, Thomas A. ;

Friez, Michael J. .

GENETIC TESTING AND MOLECULAR BIOMARKERS, 2012, 16 (06) :465-470

[4] A Dual-Mode Single-Molecule Fluorescence Assay for the Detection of Expanded CGG Repeats in Fragile X Syndrome [J].

Cannon, Brian ;

Pan, Cynthia ;

Chen, Liangjing ;

Hadd, Andrew G. ;

Russell, Rick .

MOLECULAR BIOTECHNOLOGY, 2013, 53 (01) :19-28

[5] Developing luminescent silver nanodots for biological applications [J].

Choi, Sungmoon ;

Dickson, Robert M. ;

Yu, Junhua .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (05) :1867-1891

[6] Fluorescent silver nanoclusters [J].

Diez, Isabel ;

Ras, Robin H. A. .

NANOSCALE, 2011, 3 (05) :1963-1970

[7] Naphthyridine tetramer with a pre-organized structure for 1:1 binding to a CGG/CGG sequence [J].

Dohno, Chikara ;

Kohyama, Izumi ;

Hong, Changfeng ;

Nakatani, Kazuhiko .

NUCLEIC ACIDS RESEARCH, 2012, 40 (06) :2771-2781

[8] VARIATION OF THE CGG REPEAT AT THE FRAGILE-X SITE RESULTS IN GENETIC INSTABILITY - RESOLUTION OF THE SHERMAN PARADOX [J].

FU, YH ;

KUHL, DPA ;

PIZZUTI, A ;

PIERETTI, M ;

SUTCLIFFE, JS ;

RICHARDS, S ;

VERKERK, AJMH ;

HOLDEN, JJA ;

FENWICK, RG ;

WARREN, ST ;

OOSTRA, BA ;

NELSON, DL ;

CASKEY, CT .

CELL, 1991, 67 (06) :1047-1058

[9] Highly Sequence-Dependent Formation of Fluorescent Silver Nanoclusters in Hybridized DNA Duplexes for Single Nucleotide Mutation Identification [J].

Guo, Weiwei ;

Yuan, Jipei ;

Dong, Qingzhe ;

Wang, Erkang .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (03) :932-+

[10] Facile electrochemical biosensor based on a new bifunctional probe for label-free detection of CGG trinucleotide repeat [J].

He, Hanping ;

Xia, Jingping ;

Peng, Xiaoqian ;

Chang, Gang ;

Zhang, Xiuhua ;

Wang, Yafen ;

Nakatani, Kazuhiko ;

Lou, Zhaowen ;

Wang, Shengfu .

BIOSENSORS & BIOELECTRONICS, 2013, 49 :282-289

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