Genes required for mitotic spindle assembly in Drosophila S2 cells

被引：428

作者：

Goshima, Gohta

Wollman, Roy

Goodwin, Sarah S.

Zhang, Nan

Scholey, Jonathan M.

Vale, Ronald D.

Stuurman, Nico

机构：

[1] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA 94158 USA

[3] Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA

[4] Marine Biol Lab, Physiol Course, Woods Hole, MA 02543 USA

来源：

SCIENCE | 2007年 / 316卷 / 5823期

关键词：

D O I：

10.1126/science.1141314

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

引用

页码：417 / 421

页数：5

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