Identification of Barley (Hordeum vulgare L.) Autophagy Genes and Their Expression Levels during Leaf Senescence, Chronic Nitrogen Limitation and in Response to Dark Exposure

被引:26
作者
Avila-Ospina, Liliana [1 ]
Marmagne, Anne [1 ]
Soulay, Fabienne [1 ]
Masclaux-Daubresse, Celine [1 ]
机构
[1] INRA AgroParisTech, ERL CNRS 3559, UMR1318, Inst Jean Pierre Bourgin,Saclay Plant Sci, F-78000 Versailles, France
来源
AGRONOMY-BASEL | 2016年 / 6卷 / 01期
关键词
senescence; dark stress; autophagy; nitrogen remobilization; barley; nitrogen use efficiency; SELECTIVE AUTOPHAGY; TERMINAL DOMAIN; ARABIDOPSIS; PROTEIN; YEAST; ATG8; STRESS; REMOBILIZATION; TRANSCRIPTS; METABOLISM;
D O I
10.3390/agronomy6010015
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Barley is a cereal of primary importance for forage and human nutrition, and is a useful model for wheat. Autophagy genes first described in yeast have been subsequently isolated in mammals and Arabidopsis thaliana. In Arabidopsis and maize it was recently shown that autophagy machinery participates in nitrogen remobilization for grain filling. In rice, autophagy is also important for nitrogen recycling at the vegetative stage. In this study, HvATGs, HvNBR1 and HvATI1 sequences were identified from bacterial artificial chromosome (BAC), complementary DNA (cDNA) and expressed sequence tag (EST) libraries. The gene models were subsequently determined from alignments between genome and transcript sequences. Essential amino acids were identified from the protein sequences in order to estimate their functionality. A total of twenty-four barley HvATG genes, one HvNBR1 gene and one HvATI1 gene were identified. Except for HvATG5, all the genomic sequences found completely matched their cDNA sequences. The HvATG5 gene sequence presents a gap that cannot be sequenced due to its high GC content. The HvATG5 coding DNA sequence (CDS), when over-expressed in the Arabidopsis atg5 mutant, complemented the plant phenotype. The HvATG transcript levels were increased globally by leaf senescence, nitrogen starvation and dark-treatment. The induction of HvATG5 during senescence was mainly observed in the flag leaves, while it remained surprisingly stable in the seedling leaves, irrespective of the leaf age during stress treatment.
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页数:18
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共 51 条
[1]   PROTEIN DATABASE SEARCHES FOR MULTIPLE ALIGNMENTS [J].
ALTSCHUL, SF ;
LIPMAN, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (14) :5509-5513
[2]   COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].
ARNON, DI .
PLANT PHYSIOLOGY, 1949, 24 (01) :1-15
[3]   The identification of new cytosolic glutamine synthetase and asparagine synthetase genes in barley (Hordeum vulgare L.), and their expression during leaf senescence [J].
Avila-Ospina, Liliana ;
Marmagne, Anne ;
Talbotec, Joel ;
Krupinska, Karin ;
Masclaux-Daubresse, Celine .
JOURNAL OF EXPERIMENTAL BOTANY, 2015, 66 (07) :2013-2026
[4]   Autophagy, plant senescence, and nutrient recycling [J].
Avila-Ospina, Liliana ;
Moison, Michael ;
Yoshimoto, Kohki ;
Masclaux-Daubresse, Celine .
JOURNAL OF EXPERIMENTAL BOTANY, 2014, 65 (14) :3799-3811
[5]   Variations on a theme: plant autophagy in comparison to yeast and mammals [J].
Avin-Wittenberg, Tamar ;
Honig, Arik ;
Galili, Gad .
PROTOPLASMA, 2012, 249 (02) :285-299
[6]   ULTRASTRUCTURAL ANALYSIS OF THE AUTOPHAGIC PROCESS IN YEAST - DETECTION OF AUTOPHAGOSOMES AND THEIR CHARACTERIZATION [J].
BABA, M ;
TAKESHIGE, K ;
BABA, N ;
OHSUMI, Y .
JOURNAL OF CELL BIOLOGY, 1994, 124 (06) :903-913
[7]   High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation [J].
Breeze, Emily ;
Harrison, Elizabeth ;
McHattie, Stuart ;
Hughes, Linda ;
Hickman, Richard ;
Hill, Claire ;
Kiddle, Steven ;
Kim, Youn-sung ;
Penfold, Christopher A. ;
Jenkins, Dafyd ;
Zhang, Cunjin ;
Morris, Karl ;
Jenner, Carol ;
Jackson, Stephen ;
Thomas, Brian ;
Tabrett, Alexandra ;
Legaie, Roxane ;
Moore, Jonathan D. ;
Wild, David L. ;
Ott, Sascha ;
Rand, David ;
Beynon, Jim ;
Denby, Katherine ;
Mead, Andrew ;
Buchanan-Wollaston, Vicky .
PLANT CELL, 2011, 23 (03) :873-894
[8]   The ATG Autophagic Conjugation System in Maize: ATG Transcripts and Abundance of the ATG8-Lipid Adduct Are Regulated by Development and Nutrient Availability [J].
Chung, Taijoon ;
Suttangkakul, Anongpat ;
Vierstra, Richard D. .
PLANT PHYSIOLOGY, 2009, 149 (01) :220-234
[9]   The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana [J].
Doelling, JH ;
Walker, JM ;
Friedman, EM ;
Thompson, AR ;
Vierstra, RD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (36) :33105-33114
[10]   In vitro reconstitution of plant ATG8 and ATG12 conjugation systems essential for autophagy [J].
Fujioka, Yuko ;
Noda, Nobuo N. ;
Fujii, Kiyonaga ;
Yoshimoto, Kohki ;
Ohsumi, Yoshinori ;
Inagaki, Fuyuhiko .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (04) :1921-1928